高密度脂蛋白对3T3-L1脂肪细胞分泌白细胞介素8的影响

分别用不同浓度高密度脂蛋白(HDL,0、10、50和100μg/ml)孵育3T3-L1脂肪细胞16 h,再加入100 ng/ml脂多糖共同孵育6 h.用酶联免疫吸附法(ELISA)检测各组脂肪细胞培养液中的白细胞介素8水平,半定量逆转录多聚酶链式反应(RT-PCR)测定脂肪细胞PPARγmRNA的表达.结果 显示,脂多糖刺激使脂肪细胞分泌白细胞介素8增加(P＜0.05).不同浓度HDL干预的脂肪细胞分泌的白细胞介素8水平均低于脂多糖刺激组,并且呈剂量依赖性.不同浓度HDL干预的脂肪细胞PPARγmRNA表达较单用脂多糖刺激组显著升高.这些结果提示HDL可能通过上调PPARγ的表达、抑制脂肪细胞分泌白细胞介素8分泌.

Effect of high-density lipoprotein on interleukin-8 secretion in 3T3-L1 adipocytes

3T3-L1 adipocytes were cultured with various concentrations of high-density lipoprotein ( HDL, 0, 10, 50, and 100 μg/ml ) for 16 h and with lipopolysaccharide ( LPS, 100 ng/ml ) for another 6 h. Interleukin-8 in the medium was determined by ELISA, and PPAR-γ mRNA expression by reverse transacription polymerase chain reaction (RT-PCR). Interleukin-8 levels were increased in LPS-treated cells ( P＜0.05 ), but decreased in HDL-treated cells in the dose-dependent manner. PPARγ mRNA expressions were increased in HDL-treated groups than those treated only with LPS. These results suggested HDL may decrease interleukin-8 secretion via up-regulating PPARγ expression in adipocytes.