单中心191例健康供者应用G-CSF动员造血干细胞的影响因素分析

本研究旨在探讨粒细胞集落刺激因子（G-CSF）动员外周血造血干细胞的影响因素及对健康供者的影响。181例志愿健康供者应用G-CSF5-10μg/（kg·d）动员，10例应用G-CSF3．3-4．9μg/（kg·d）动员，12h1次，连续动员4-5d；采集、检测外周血中单个核细胞（MNC）数与CD34＋细胞数，并观察供者动员及采集过程中的不良反应。结果表明，与动员前相比，动员后（采集前）供者外周血白细胞数平均升高7倍（P〈0．01）；血小板数明显下降（P〈0．01）；血红蛋白含量无明显差异性。动员第4或5天采集效果无差异。男性供者采集的MNC、CD34＋细胞数高于女性（P〈0．01），高体重供者采集的MNC、CD34＋数高于低体重供者，年龄对采集效果无明显影响。G-CSF剂量与采集效果无线性关系。供者不良反应轻微。结论：G-CSF可以有效动员外周血造血干细胞，供者无明显的不良反应。

Analysis of Influence Factors on G-CSF-Mobilized Hematopoietic Stem Cells of 191 Healthy Donors in a Monocenter

This study was aimed to investigate the factors influencing mobilization efficiency of peripheral hematopoi- etic stem cells with granulocyte colony stimulating factor （G-CSF） and their impact on healthy donors. 181 donors were mobilized subcutaneously with G-CSF at 5 -10 μg/（kg·d）, and 10 donors were mobilized with G-CSF at 3.3 -4.9μg/（kg·d）, once 12 h, for4 -5 d. Peripheral blood mononuclear cell （MNC） and CD34＋cell counts were analyzed by flow cytometry. Mobilization-related side effects were also monitored. The results showed that white blood cell counts increased by 6 times averagely after mobilization （ P 〈 0.01 ）. The platelet count obviously decreased （ P 〈 0. 01 ）, while the hemoglobin level did not show significant difference. No significant differences were observed in MNC and CD34 ＋ cell counts between those subjects harvested on the 4th and 5th day after mobilization. Male donors were superior to female ones in cell harvest（ P 〈 0.01 ）. Donor body weight played positive role in cell yield, while impact of age on harvest was not remarkable. Neither MNC nor CD34 ＋ cell count showed a linear relationship with G-CSF dose. Only slight side effects were observed on the donors in this study. It is concluded that mobilization with G-CSF is sufficient in healthy donors without remarkable side effects.