| 藤黄酸对SKM-1细胞生长抑制作用及其机制 |
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| 引用本文： | 孙耘玉,王淑君,陈宝安,夏国华,丁家华,高冲,宋慧慧,郭青龙,张海伟.藤黄酸对SKM-1细胞生长抑制作用及其机制[J].中国实验血液学杂志,2013,21(1):105-109. |
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| 作者姓名： | 孙耘玉 王淑君 陈宝安 夏国华 丁家华 高冲 宋慧慧 郭青龙 张海伟 |
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| 作者单位： | 1. 东南大学医学院附属中大医院血液科/MDS研究所,江苏南京,210009
2. 中国药科大学江苏省肿瘤发生与干预重点实验室,江苏南京,210009 |
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| 基金项目： | 国家科技支撑计划课题(十一五)子课题，江苏省医学重点学科 |
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| 摘 要： | 本研究探讨藤黄酸（GA）对骨髓增生异常综合征细胞SKM-1生长抑制和诱导细胞凋亡及其机制。采用MTr比色法检测GA对SKM-1细胞的增殖抑制作用，在光学显微镜下观察细胞形态学变化，流式细胞术检测细胞凋亡和细胞周期分布，RT-PCR检测SKM-1细胞baxmRNA和bcl-2mRNA表达情况。结果表明：GA能明显抑制SKM-1细胞增殖，具有时间和剂量依赖性，其48h的IC50值为0．37μg/ml；GA诱导SKM-1细胞凋亡，具有浓度依赖性，诱导SKM-l阻滞在G0／G1期；显微镜下可见典型的凋亡细胞形态特征；GA可明显下调SKM-1细胞bcl-2mRNA表达和上调baxmRNA表达。结论：GA能诱导SKM-1细胞凋亡，其机制可能与细胞G0／G1期阻滞及bcl-2／bax比率下调有关。
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| 关 键 词： | SKM-1细胞 MDS 藤黄酸 凋亡 |
Effect of Gambogic Acid on Proliferation of SKM-1 Cells and Its Mechanism |
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| SUN Yun-Yu ,WANG Shu-Jun ,CHEN Bao-An ,XIA Guo-Hua,DING Jia-Hua,GAO Chong,SONG Hui-Hui,G UO Qing-Long,ZHANG Hai-Wei,LI Chun-Rui,ZHOU Jian-Feng.Effect of Gambogic Acid on Proliferation of SKM-1 Cells and Its Mechanism[J].Journal of Experimental Hematology,2013,21(1):105-109. |
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| Authors: | SUN Yun-Yu WANG Shu-Jun CHEN Bao-An XIA Guo-Hua DING Jia-Hua GAO Chong SONG Hui-Hui G UO Qing-Long ZHANG Hai-Wei LI Chun-Rui ZHOU Jian-Feng |
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| Institution: | 2 Department of Hematology,Zhongda Hospital,Southeast University Medical School,Nanjing 210009,Jiangsu Province,China;1 Jiangsu Key Laboratory of Carcinogenesis and Intervention,China Pharmaceutical University,Nanjing 210009,Jiangsu Province,China;2 Department of Hematology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China |
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| Abstract: | The aim of this study was to explore the effect of gambogic acid （GA） on MDS SKM-1 cell proliferention, apoptosis and their possible mechanism. Cell proliferation was determined by MTT method. The apoptosis percentage and cell cycle regulation of SKM-1 cells were analyzed by flow cytometry. Morphological features were observed by light microscopy. The mRNA expression of bcl-2 and bax were detected by RT-PCR. The results showed that GA could inhibit the proliferation of SKM-1 cells in a dose- and time-dependent manner （ IC50 was 0.37 μg/ml at 48 h）, increase the apoptotic percentage of SKM-1 cells,and arrest cell cycle at the G0/G1. The expression of bax mRNA was up-regulated while that of bcl-2 mRNA was down-regulated in SKM-1 cells treated with GA for 48 h. It is concluded that GA can induce apoptosis, which may be related to its effect of arresting cells at phase of G0/G1 and down-regulating bcl-2/bax ratio. |
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| Keywords: | SKM-1 cell MDS gambogic acid apoptosis |
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