穴位注射胞二磷胆碱对创伤性脑损伤大鼠神经功能障碍的影响

目的：观察足三里穴位注射胞二磷胆碱疗法对创伤性脑损伤大鼠神经功能的影响。方法：成年健康雄性SD大鼠40只，采用改进的Feeney法建立大鼠脑损伤模型，设假手术组大鼠8只（A组），造模成功大鼠32只随机分为穴位注药组（B组）、穴位注水组（c组）、腹腔给药组（D组）和对照组（E组）各8只。造模后每日B、C、D、E组分别给予足三里穴位注射胞二磷胆碱及生理盐水、腹腔注射胞二磷胆碱及生理盐水处理，连续14d。采用神经功能缺损评分、斜板试验及平衡试验观察造模后第1、2、4、6、8、10、12、14天各处理方法对大鼠的神经行为的影响。结果：致伤后第1天B、C、D、E组大鼠神经行为学评分及平衡能力评分均较致伤前及A组明显增加（P〈O．05）。伤后评分逐渐下降，B组大鼠得分除第1天外均低于c、D、E组（P〈0．05），C、D、E组各时间点比较评分差异无统计学意义。致伤后B、C、D、E组爬坡角度均较致伤前及A组明显下降（P〈0．05），B组大鼠在致伤第2天开始爬坡角度明显大于C、D、E组（P〈0．05）。结论：穴位注射胞二磷胆碱能明显促进脑创伤大鼠神经功能的恢复。

Effects of the citicoline acupuncture point injection on neural dysfunction after brain trauma in rats

Objective：To investigate the effects of injection of citicoline into Zusanli point on neural dysfunction of rats following traumatic brain injury. Methods：Forty healthy adult male Sprague-Dawley （SD） rats were randomly divided into five groups： sham-operated group （A）, acupuncture point drug injection group （B）, acupuncture point saline injection group （C） ,intraperitoneal drug injection group （D） and control group （E）. Opened brain trauma was induced by the improved Feeney method in the 32 rats of groups B,C,D and E. Eight sham-operated rats were sub- jected to the same surgical procedure,except brain injury. The rats in groups B and C were treated with acupuncture injection of citieoline or saline daily respectively. The rats in groups D and E were treated with intraperitonea injec- tion of citicoline or saline daily respectively. The treatment continued for 14 days. On the lst,2nd,4th,6th,8th, 10th, 12th and 14th day after injury, the alteration of neural function in rats was assessed by using neurological severity score,balance test and slanting board test. Results： The neurological severity score and the balance test score in groups B,C,D and E were increased significantly as compared with those before injury and in group A on the 1st day after injury （P〈0.05） ,then gradually decreased. The scores in group B were lower than in groups C,D and E except the 1st day after injury （P〈0. 05）. The inclined board angle in groups B, C,D and E was decreased markedly as compared with that before injury and in group A （P〈0.05）. The inclined board angle in group B was more than that in groups C, D and E from the 2nd day after injury （P〈0.05）. Conclusion：Acupuncture point drug injection therapy could significantly promote the recovery of neural dysfunction after traumatic brain injury.