| 大蒜素诱导骨肉瘤细胞凋亡的作用及其机制的实验研究 |
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| 引用本文： | 张小虎,唐超雄,陈小华,邓钦民,雷振衡,曾志华,李有秋.大蒜素诱导骨肉瘤细胞凋亡的作用及其机制的实验研究[J].肿瘤药学,2012(3):180-183. |
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| 作者姓名： | 张小虎 唐超雄 陈小华 邓钦民 雷振衡 曾志华 李有秋 |
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| 作者单位： | 湖南省常宁市中医医院;湖南师范大学医学院 |
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| 基金项目： | 湖南省教育厅基金项目资助(09C632) |
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| 摘 要： | 目的探讨大蒜素诱导骨肉瘤细胞凋亡的作用及其机制。方法使用不同浓度大蒜素处理骨肿瘤细胞系后,采用流式细胞术检测细胞凋亡率,RT-PCR技术以及免疫印迹技术检测Bax的mRNA及蛋白水平的表达变化。结果大蒜素处理后骨肉瘤细胞凋亡率明显升高,并呈剂量依赖性;且细胞内的Bax的mRNA及蛋白表达均显著升高(P<0.05)。结论大蒜素在体外可诱导多种骨肉瘤细胞凋亡,可能与上调Bax的表达,促进细胞色素c的释放有关;大蒜素有望成为一种新的治疗骨肉瘤的化疗药物。
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| 关 键 词： | 骨肉瘤 大蒜素 凋亡 Bax |
Effects and Mechanisms of Osteosarcoma Cell Apoptosis Induced by Allicin |
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| Zhang Xiaohu,Tang Chaoxiong,Chen Xiaohua,Deng Qinmin,Lei Zhenheng,Zeng Zhihua,Li Youqiu.Effects and Mechanisms of Osteosarcoma Cell Apoptosis Induced by Allicin[J].Anti-Tumor Pharmacy,2012(3):180-183. |
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| Authors: | Zhang Xiaohu Tang Chaoxiong Chen Xiaohua Deng Qinmin Lei Zhenheng Zeng Zhihua Li Youqiu |
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| Institution: | 1Changning City Chinese Medicine Hospital of Hunan Province,Changning,Hunan,421500,China,2Hunan Normal University School of Medicine,Changsha,Hunan,410013,China) |
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| Abstract: | Objective To investigate the effects and mechanisms of osteosarcoma cell apoptosis induced by allicin.Methods Osteosarcoma cells were treated with different concentrations of allicin in vitro,the apoptotic rate was detected by flow cytometry,mRNA and protein expression of Bax were detected respectively by using RT-PCR and western blotting.Results The apoptotic rate of osteosarcoma cells was significantly increased in a dose-dependent manner after being treated with allicin.Both mRNA and protein expressions of Bax in osteosarcoma cells increased significantly(P<0.05).Conclusion Allicin was able to induce the apoptosis of osteosarcoma cells through upregulating the expression of Bax in vitro.It may become a potential chemotherapeutic agent for the treatment of osteosarcoma. |
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| Keywords: | Osteosarcoma Allicin Apoptosis Bax |
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