移植前低钾右旋糖苷液二次灌洗减轻供肺再灌注损伤

目的观察移植前低钾右旋糖苷液(LPD)二次灌洗对供肺再灌注损伤的保护作用。方法24只大鼠随机分为对照组(n=12)和实验组(n=12),对照组供肺经LPD液灌洗后于4℃保存18 h,取右肺组织测丙二醛(MDA)、髓过氧化物酶(MPO)、白细胞介素-8(IL-8),建立离体肺再灌注模型,左肺灌注30 m in;实验组供肺灌注前用LPD液再灌洗一次,余同对照组。灌注期间每隔10m in测定供肺静脉流出血氧分压(PaO2)、供肺平均肺动脉压(PaP)、气道峰压(PawP),灌注结束后测肺组织丙二醛(MDA)、髓过氧化物酶(MPO)、白细胞介素-8(IL-8)的含量。结果实验组供肺右肺组织MDA、MPO、IL-8含量明显低于对照组,差异有统计学意义(P<0.01);再灌注30m in后实验组供肺PO2明显高于对照组,PaP、PawP明显低于对照组,差异有统计学意义(P<0.01);灌注后供肺组织MDA、MPO、IL-8含量,实验组明显低于对照组,差异有统计学意义(P<0.01)。结论移植前LPD液二次灌洗可以减少供肺组织内MDA、MPO、IL-8含量,减轻供肺的再灌注损伤,改善供肺的功能。

Administering a second rinse solution of low-potassium-dextran before reperfusion ameliorates the donor lung

Objective To studied the effects of administering a second rinse solution of low-potassium-dextran(LPD) before reperfusion on the donor lung of rat after long time,cold ischemic preservation.Methods Twenty-four rats were randomly divided into control group(n=12) and trial group(n=12).Donor lungs of control group were flushed with LPD solution.The levels of malonaldehyde(MDA),myeloperoxidase(MPO) and interleukin-8(IL-8) were tested in the tissue of right lung and the left lungs were reperfused with venous blood for 30 minutes.The partial pressure of oxygen(PaO_2),PaP and PawP were measured at every 10 minutes intervals during reperfusion.After reperfusion,the MDA,MPO activity and interlukin-8(IL-8) were determined in the lung tissue.Results The levels of MDA,MPO and IL-8 decreased significantly in right lung of trial group than those in control group(P<0.01).After reperfusion of 20 minutes,the PaO_2 were higher in trial group than that in control group,and PaP,PawP were lower in trial group than those of control group(all P<0.01).After reperfusion,the MDA,MPO and IL-8 decreased significantly in left lung of trial group than in control group(all P<0.01).Conclusion A second flush with LPD solution before reperfusion can decrease the levels of MDA,MPO and IL-8,and thus improves the function of donor lungs.