血必净注射液对烫伤大鼠肺组织高迁移率族蛋白B1表达的影响及意义

目的 观察中药血必净注射液(简称血必净)对烫伤大鼠肺组织高迁移率族蛋白B1(HMGB1)表达及炎症反应的影响.方法 采用大鼠30% TBSA三度烫伤延迟复苏模型.78只雄性Wistar大鼠随机分为假烫伤组(n=18)、烫伤组(n=30,伤后2h静脉注射生理盐水4ml/kg)和血必净组(n=30,伤后2h静脉注射血必净4ml/kg),于伤后8、24、72h活杀动物,留取肺组织,采用RT-PCR和Western blot检测HMGB1基因和蛋白的表达,酶学分光光度法测定髓过氧化物酶(MPO)活性.结果 与假烫伤组比较,烫伤组肺组织HMGB1基因和蛋白表达在伤后8～72h显著增强(P＜0.05或0.01),MPO活性在伤后8h及24h明显增高(P＜0.01).与烫伤组比较,血必净组大鼠肺组织HMGB1表达在伤后24h及72h显著下调(P＜0.05或0.01),MPO活性在24h时间点显著降低(P＜0.01).结论 HMGB1作为晚期炎症因子参与了烫伤后肺组织炎症反应的病理过程,血必净治疗可明显下调肺组织HMGB1表达,并减轻烫伤延迟复苏所致急性肺损伤.

Effect of XueBiJing injection on the expression of high mobility group box-1 protein in lung of rats with burn injury

Objective To investigate the effect of XueBiJing injection(a concoction of Chinese herbs)on the expression of high mobility group box-1 protein(HMGB1)in the lung with acute lung injury of rats following delayed resuscitation after burn injury.Methods 78 male rats were randomly divided into sham scald group(n=18),scald group(n=30)and XueBiJing treatment group(n=30).In the scald group and XueBiJing treatment group,4ml/kg normal saline or XueBiJing was intravenously injected 2 hours after scald injury.In the latter two groups,rats were subjected to 30% TBSA full-thickness scald injury,and fluid resuscitation was delayed.The animals were sacrificed at 8h,24h and 72h after injury,respectively.Lung tissue samples were collected to determine the expression of HMGB1 mRNA and protein,and the activity of pulmonary myeloperoxidase(MPO).HMGB1 mRNA level was semi-quantitatively measured by RT-PCR using GAPDH as an internal standard,and protein expression of HMGB1 was determined by Western blot.Results Compared with sham scald controls,both mRNA and protein expressions of HMGB1 were significantly enhanced in the lung at 8-72h after scald injury(P<0.05 or P<0.01),meanwhile the pulmonary MPO activities were markedly increased at 8h and 24h after scald(P<0.01).Treatment with XueBiJing could markedly down-regulate the expressions of pulmonary HMGB1 mRNA and protein at 24h and 72h,and reduced pulmonary MPO activity at 24h following scald(P<0.05 or P<0.01).Conclusion HMGB1 appears to be involved in the pathogenesis of post burn acute lung injury.Treatment with XueBiJing injection could obviously inhibit pulmonary HMGB1 expression and reduce MPO activity in rats with delayed resuscitation after scald injury.