大孔吸附树脂纯化黄花草木犀的工艺研究

目的考察黄花草木犀中芦丁、clovin和香豆素的最佳大孔吸附树脂的纯化工艺。方法通过对不同型号大孔树脂的考察优选出纯化黄花草木犀中药效成分的最佳树脂类型,并对其最大上样量、上样体积流量、洗脱溶剂体积分数和洗脱溶剂最佳用量进行优化。结果经过筛选确定HPD-300大孔树脂为纯化黄花草木犀药效成分的最优树脂类型,其最佳纯化条件:树脂的上样量为2.4 g生药/g树脂,上样体积流量为2.0 BV/h,依次用2 BV水洗脱和7 BV 50%乙醇洗脱。在此条件下芦丁、clovin、香豆素质量分数提升了7.8倍,平均收率为85.38%。结论 HPD-300大孔树脂用于纯化黄花草木犀中芦丁、clovin、香豆素稳定性高、重复性好。

Purification of Melilotus officinalis by macroporous resins

Objective To investigate the optimum purification process of the active ingredients rutin, clovin, and coumarin in Melilotus officinalis by macroporous resins. Methods The various types of resins were selected with purification of the active ingredients rutin, clovin, and coumarin in M. officinalis as index. The parameters, such as sample loading maximum volume, sample loading velocity, elution solvent concentration, and elution solvent volume of optimized resin were studied. Results HPD-300 type macroporous resin was selected as the best type of resin for the purification of active ingredients in M. officinalis. The optimum purification conditions were as following: the sample loading maximum volume was 2.4 g/g macroporous resin, sample loading velocity was 2.0 BV/h, and the sample was eluted by water with 2 BV and 50% ethanol with 7 BV, respectively. Rutin, clovin and coumarin proportion promoted 7.8 times after the purification HPD-300 by macroporous resin, and the yield was 85.38%. Conclusion HPD-300 macroporous resin is suitable for the purification of rutin, clovin, and coumarin in M. officinalis with high stability and good repeatability.