| 酪氨酸激酶抑制剂STI571对K562细胞周期及相关基因表达的研究 |
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| 引用本文： | 李婉红,陈琪,王伶俐.酪氨酸激酶抑制剂STI571对K562细胞周期及相关基因表达的研究[J].现代免疫学,2010(1). |
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| 作者姓名： | 李婉红 陈琪 王伶俐 |
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| 作者单位： | 浙江省台州市中心医院检验中心;温州医学院第二附属医院检验科; |
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| 摘 要： | 为了探讨不同浓度STI571对K562细胞ATM(ataxia telangiectasia mutated)/ATR(ATM-Rad3-Related)基因表达的影响及相应的细胞周期变化和细胞凋亡机制。以不同剂量的STI571作用于K562细胞株,通过联苯胺、吉姆萨2瑞氏染色和流式细胞术证实其分化方向,RT-PCR半定量检测ATM/ATR基因表达,流式细胞术检测BCL-2?Annexin-V和细胞周期。结果随药物浓度升高,K562细胞向红系分化明显,ATM基因表达量增强而ATR基因表达量降低,Bcl-2水平下降,细胞凋亡显著,亚二倍体和G1期细胞数增多,S期和G2/M期细胞减少。不加入药物的K562细胞ATM和ATR几乎不表达。ATM和ATR基因表达水平的变化呈现STI571浓度依赖形式,并与细胞周期改变、细胞凋亡和红系分化相关。
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| 关 键 词： | 酪氨酸激酶抑制剂 STI571 ATM/ATR基因 细胞周期变化 |
Effect of protein kinase inhibitor STI571 on the growth and apoptosis of K562 cells and the expression of the ATM/ATR gene |
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| LI Wan-hong,CHEN Qi,WANG Ling-li.Effect of protein kinase inhibitor STI571 on the growth and apoptosis of K562 cells and the expression of the ATM/ATR gene[J].Current Immunology,2010(1). |
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| Authors: | LI Wan-hong CHEN Qi WANG Ling-li |
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| Institution: | LI Wan-hong1,CHEN Qi1,WANG Ling-li2(1.Laboratory,Taizhou Central Hospital of Zhejiang Provience,Taizhou 318000,China,2.Laboratory of the Second Affiliated Hospital Wenzhou Medical College,Wenzhou 325000,China) |
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| Abstract: | To investigate the effects of protein kinase inhibitor STI571 on the ATM/ATR mRNA expression and the corresponding dynamic changes of cell cycle and mechanisms of apoptosis erythyroid-leukemia cell line K562 cells were treated with various concentration of STI571 and the cell differentiation model were evaluated by the staining assay of benzidine and Wright-Giemsa and fluorescence activivated cell analyzer.RT-PCR was introduced to semi-quantify the ATM/ATR mRNA expression and flow cytometry was applied to m... |
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| Keywords: | protein kinase inhibitor STI571 ATM/ATR gene cell cycle change erythroid differentiation |
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