异氟烷和七氟烷对缺血神经元c-fos和bcl-2基因表达的影响及神经保护作用

为了解吸入麻醉药异氟烷和七氟烷对缺血神经元的保护作用和对c fos、bcl 2基因表达的影响 ,将大鼠右大脑中动脉和双侧颈总动脉分别结扎 1h,造成脑缺血再灌模型 ,缺血期间分别吸入异氟烷和七氟烷。然后分别检测脑梗死体积、海马组织神经元凋亡变化和c fos、bcl 2基因表达情况。结果显示 :缺血期间吸入麻醉药组脑梗死体积、凋亡神经元细胞数和c fosmRNA表达水平显著低于缺血组 ,而bcl 2mRNA表达水平吸入麻醉药组和缺血组之间没有明显差别。表明异氟烷和七氟烷对缺血神经元有保护作用 ,能衰减c fosmRNA表达 ,但对bcl 2mRNA的表达无明显影响。

Effects of Isoflurane and Sevoflurane on c-fosand bcl-2 Genes Expression in Ischemic Neuron and Neuroprotective Role

To study the effects of isoflurane and sevoflurane on c-fos and bcl-2 genes expression and protective in ischemic neuron, cerebral ischemia was produced by occlusion of right middle cerebral artery (MCA) and bilateral common carotid arteries (CCAs) for 1 h in rats. The rats were inhaled isoflurane and sevoflurane respectively at 1.0 minimum alveolar concentration during the ischemia. The infarct volume was delineated by triphenyltetrazolium chloride (TTC) stain. The DNA damage was detected by in situ end-labeling staining method on brain section. The expression of c-fos and bcl-2 genes were determined by in situ hybridization on brain section. Infarct volume and apoptotic cell of inhaled anesthetic groups were significant reduced compared with ischemic group at 72 h after 1 h of MCA/CCAs occlusion. The relative levels of c-fos mRNA induction of the inhaled anesthetic groups were significantly lower in the hippocampus at 4 h after 1 h of MCA/CCAs occlusion than that of the ischemic group. There was no statistical difference in bcl-2 mRNA relative levels between the inhaled anesthetic groups and the ischemic group in hippocampus at 24 h after 1 h of MCA/CCAs occlusion. These results shows that the isoflurane and sevoflurane have protective effects in ischemic neuron. For animals that inhaled isoflurane or sevoflurane during ischemia there was attenuation of c-fos mRNA induction in hippocampus, but there was no effect on bcl-2 mRNA expression.