白藜芦醇抑制胃癌BGC823细胞增殖机制的研究

目的观察白藜芦醇(Res)抑制人胃癌BGC823细胞增殖的影响。方法采用MTT法、集落形成实验、流式细胞仪(FCM)和检测端粒酶活性来观察Res作用于人胃癌BGC823细胞48h后对肿瘤细胞增殖和细胞周期的影响。结果1.5、3.0、6.0、12.0、24.0mg/L的Res对人胃癌BGC823细胞生长抑制率分别为19.1%、25.3%、52.6%、67.9%、91.3%,Res处理后BGC823细胞集落形成明显受到抑制,使细胞周期阻滞于S期,端粒酶活性受到抑制。结论Res对人胃癌BGC823细胞的增殖抑制作用明显。

The mechanisms of inhibition in proliferation of the human gastric cancer cell line BGC823 cells by resveratrol

Objective To explore the mechanisms of resveratrol which inhibiti proliferation of the human gastric carcinoma cell line BGC823 cells. Methods By MTT assay and flow cytometry (FCM) were respectively used to determine the influence of resveratrol which affected the gastric carcinoma cell line BGC823 cells in its proliferation and cell cycle for 48 hours. Results The inhibitive rates of gastric carcinoma cell line BGC823 were 19.1%, 25.3%, 52.6%, 67.9% and 91.3% with 1.5,3.0,6.0,12.0,24.0 mg/L concentration of resveratrol respectively. The clone formation of BGC823 cell was evidently inhibited and cell cycle was arrested in S-phase and inhibited telomerase activity after it was treated with resveratrol. Conclusion The effect of resveratrol which inhibited the proliferation of the human gastric carcinoma cell line BGC823 was related with S-phase arresting of cell cycle.