| 不同诱变方法选育高产G-418菌株的比较 |
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| 引用本文： | 钟艾玲 田敏,刘艳全 易欣 龙燕 雷叶明 王富强 刘亚洲.不同诱变方法选育高产G-418菌株的比较[J].中国抗生素杂志,2019,44(4):422-425. |
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| 作者姓名： | 钟艾玲 田敏 刘艳全 易欣 龙燕 雷叶明 王富强 刘亚洲 |
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| 作者单位： | 抗生素研究与再评价四川省重点试验室;微生物药物生物合成技术国家地方联合工程研究中心 |
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| 基金项目： | 四川省科技计划应用基础研究项目(No.2017JY0256);四川省医药微生物共享服务平台建设(No.2018TJPT004) |
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| 摘 要： | 目的选育抗生素G-418高产的棘孢小单孢菌突变株,以提高抗生素G-418的产量。方法采用紫外诱变、NTG诱变、微波诱变、超声诱变的方法对棘孢小单孢菌进行诱变处理,结合生物活性测定方法,以发酵单位为主要评价标准进行筛选。结果获得两株G-418高产菌株,其产量比出发菌株分别提高了102%和115%。结论本研究表明紫外诱变可以显著提高G-418产生菌的正突变率,尤其是种子液衰亡期的正突变率最高,为选育G-418棘孢小单孢高产菌株奠定了基础。
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| 关 键 词： | 诱变方法 衰亡期 选育 |
Study on mutation methods to screen high yield G-418 strains |
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| Zhong Ai-ling,Tian Min,Liu Yan-quan,Yi Xin,Long Yan,Lei Ye-ming,Wang Fu-qiang,Liu Ya-zhou.Study on mutation methods to screen high yield G-418 strains[J].Chinese Journal of Antibiotics,2019,44(4):422-425. |
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| Authors: | Zhong Ai-ling Tian Min Liu Yan-quan Yi Xin Long Yan Lei Ye-ming Wang Fu-qiang Liu Ya-zhou |
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| Institution: | (Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052;National Engineering Research Center for Microbial Drug Biosynthesis, Chengdu Yacht Bio-Technology Co., Ltd., Chengdu 610041) |
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| Abstract: | Objective The purpose of the study is to breed the mutanted Micromonospora echinoapora which can increase the antibiotic production of G-418. Methods By means of the mutagenesis of UV, NTG, microwave, and ultrasonic, combined with the bioactivity assay, the high-yield mutants were selected based on the titre of G-418. Results Two high-yielding strains were obtained with G-418 production increased by 102% and 115%, respectively, compared to the original strain. Conclusion This study shows that UV mutagenesis can significantly increase the positive mutation rate of G-418 producing strains, and especially the seed broth in the decline phase had the highest positive mutation rate, which lays a foundation for the breeding of G-418 high-yielding strains of |
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| Keywords: | Mutation methods Decline phase Breeding |
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