早发型子痫前期胎盘细胞侵袭相关信号通路表达及其交互作用的研究

目的:探讨细胞侵袭相关Notch1及Wnt1信号通路在早发型子痫前期患者胎盘组织中的表达以及两者的交互作用。方法:收集2016年6月—2017年12月郑州人民医院收治的176例早发型子痫前期患者(轻度组102例、重度组74例),同期60例健康孕妇为对照组。收集所有研究对象分娩后的胎盘组织样本,通过免疫组织化学法检测组织Notch1及Wnt1蛋白的表达,Western blotting检测Notch1、Jagged1、Wnt1及β-catenin蛋白表达水平,并对各蛋白表达水平进行相关性分析。结果:3组Notch1及Wnt1表达强度和阳性表达率差异均有统计学意义(P<0.05);轻度组和重度组Notch1及Wnt1阳性表达率低于对照组,重度组阳性表达率低于轻度组(P<0.05)。3组Notch1、Jagged1、Wnt1及β-catenin蛋白表达水平差异有统计学意义(P<0.05);轻度组及重度组Notch1、Jagged1及Wnt1蛋白表达水平均低于对照组,重度组β-catenin表达水平低于对照组,且重度组Notch1、Jagged1及Wnt1及β-catenin均低于轻度组(均P<0.05)。Notch1与Jagged1(r=0.826,P=0.000)、Wnt1与β-catenin(r=0.531,P=0.000)表达水平呈正相关,Wnt1与Notch1(r=-0.167,P=0.010)、Wnt1与Jagged1(r=-0.136,P=0.037)表达水平呈负相关。结论:Notch1及Wnt1低表达与早发型子痫前期的发生、发展相关,且在该过程中Notch及Wnt信号通路之间可能存在一定的交互作用。

Study on the Expression of Cell-Invasion Related Signal Paths in Placenta of Early-Onset Pre-Eclampsia Patients and Their Interaction

Objective：To assess the expression of Notch1 and Wnt1 in placental tissues of early-onset pre-eclampsia patients and their interaction. Methods：176 early-onset pre-eclampsia patients were hospitalized in People′s Hospital of Zhengzhou from June 2016 to December 2017, of whom 102 were mild and 74 were severe. 60 healthy pregnant women were taken as control group. Placental tissue samples of all subjects were collected after delivery. The expressions of Notch1 and Wnt1 proteins in placentas were measured by immunohistochemistry and the levels of Notch1, Jagged1, Wnt1 and β-catenin were measured by Western blotting. The correlation of the expression of each protein were analyzed. Results：There were significant differences of Notch1 and Wnt1 expressions intensity among groups (P＜0.05), the positive rates of Notch1 and Wnt1 in the mild group and severe group were lower than those in the control group, and the positive expression rates in the severe group were lower than those in the mild group (both P＜0.05). There were statistical differences of the levels of Notch1, Jagged1, Wnt1 and β-catenin protein among groups (P＜0.05). The Notch1, Jagged1 and Wnt1 levels in the severe group and mild group were significantly lower than those in control group (all P＜0.05). The Notch1, Jagged1, Wnt1 and β-catenin expression levels in the severe group were lower than those in the mild group (all P＜0.05). Only β-catenin level in the severe group was significantly lower than that in the control group. The expressions of Notch1 and Jagged1 (r=0.826, P=0.000) and Wnt1 and β-catenin (r=0.531, P=0.000) were positively correlated, while Wnt1 and Notch1 (r=－0.167, P=0.010) and Wnt1 and Jagged1 (r= -0.136, P=0.037) were negatively correlated. Conclusions：The low expressions of Notch1 and Wnt1 were associated with the occurrence and progression of early-onset pre-eclampsia, and there may be some interaction between Notch and Wnt signaling pathways in this process.