胃癌组织中miR-98和miR-183表达及临床意义

目的   探讨微小RNA-98（miR-98）及微小RNA-183（miR-183）在胃癌组织中的表达情况及临床意义。方法   收集本院2013年1月至2016年12月90例手术切除的胃癌组织和癌旁组织标本，采用实时定量PCR（QPCR）检测miR-98和miR-183表达水平，分析其与临床病理参数（性别、年龄、淋巴结转移、分化程度、远处转移、TNM分期、浸润深度、Lauren分型和肿瘤大小）的关系，采用受试者工作特征曲线（ROC）分析两者单独和联合预测胃癌的价值，采用Pearson相关分析miR-98和miR-183表达的相关性。结果   胃癌组织中miR-98和miR-183表达水平分别为1.567±0.124和0.629±0.097，与癌旁组织比较，miR-98在胃癌组织中表达显著上调而miR-183表达下调，差异有统计学意义（P＜0.05）。胃癌组织中miR-98和miR-183表达呈负相关（r=-0.2995，P=0.004）。miR-98表达与淋巴结转移及分化程度有关，而miR-183表达与淋巴结转移、远处转移及肿瘤大小有关（P＜0.05）。ROC曲线显示，miR-98诊断胃癌的曲线下面积（AUC）为0.794，灵敏度和特异度分别为61.11％和95.56％；miR-183的AUC为0.824，灵敏度和特异度分别为76.67％和76.67％；两者联合的AUC为0.903，灵敏度和特异度分别为74.44％和91.11％。结论  miR-98在原发性胃癌中高表达而miR-183异常低表达，两者表达存在负相关并与胃癌转移、分化有关，可作为胃癌潜在的分子筛查标志物。

Expression and clinical significance of miR-98 and miR-183 in gastric cancer

Objective To explore the expression of microRNA-98 （miR-98） and microRNA-183 （miR-183） in gastric cancer and their clinical significances. Methods From January 2013 to December 2016， 90 cases of gastric cancer tissues and paired adjacent tissues were collected in our hospital. Quantitative real-time PCR （QPCR） was performed to evaluate the expression of miR-98 and miR-183. The relationships between levels of miR-98 and miR-183 with the clinicopathological parameters （sex， age， lymph node metastasis， differentiation， distant metastasis， TNM staging， depth of infiltration， Lauren typing and tumor size） were analyzed. The receiver operating characteristic curve （ROC） was used to analyze the value of both alone and combined in predicting gastric cancer. Pearson correlation analysis was used to analyze the correlation between miR-98 and miR-183 expression. Results The expression levels of miR-98 and miR-183 in cancer tissues were 1.567±0.124 and 0.629±0.097， respectively. Compared with adjacent tissues， there were upregulated levels of miR-98 but downregulated levels of miR-183 in gastric cancer tissues （P＜0.01）. Meanwhile， there was a negative correlation between miR-98 and miR-183 （r=-0.2995， P=0.004）. The expression of miR-98 was related to lymph node metastasis and differentiation， while miR-183 expression was related to lymph node metastasis， distant metastasis and tumor size （P＜0.05）. The ROC curve analyses revealed that miR-98 had considerable diagnostic accuracy， yielding an AUC （area under curve） of 0.794 with 61.11 ％ sensitivity and 95.56％ specificity in discriminating gastric cancer tissues. The AUC of miR-183 was 0.824 with the sensitivity and specificity of 76.67％ and 76.67％. The AUC of combination of miR-98 and miR-183 was 0.903 with sensitivity and specificity of 74.44％ and 91.11％. Conclusion MiR-98 is highly expressed in primary gastric cancer， while miR-183 is abnormally low， and there is a negative correlation between miR-98 and miR-183. Both miRNAs are related to metastasis and differentiation in gastric cancer. These findings indicate that miR-98 and miR-183 might serve as the potential biomarker for the detection of gastric cancer.