新疆黑种草子总黄酮体外抗宫颈癌活性研究

目的：检测新疆民族药材黑种草子总黄酮的体外抗宫颈癌活性，并初步探讨新疆黑种草子总黄酮成分的体外抗肿瘤机制。方法：以宫颈癌细胞株SiHa和HeLa为实验对象，分为空白对照组和处理组（2.5、5、10、15、20、25、50 μg/mL），使用MTT法检测不同浓度的黑种草子总黄酮体外对宫颈癌细胞株的抗增殖活性，最终确定有效药物浓度范围及半数抑制率（IC₅₀），通过划痕实验检测其对宫颈癌细胞株的抗迁移作用，使用流式细胞术分别检测对照组和处理组宫颈癌细胞凋亡率。结果：与空白对照组相比较，黑种草子总黄酮处理组SiHa和HeLa细胞的增殖均受到抑制，并呈浓度依赖性，黑种草子总黄酮对SiHa细胞株的IC₅₀为16.935 μg/mL，对HeLa细胞株的IC₅₀为18.366 μg/mL。处理前后划痕宽度比较发现，SiHa细胞株空白对照组的肿瘤细胞相对迁移距离为（223.333±13.868）px，处理组的相对迁移距离为（56.333±10.970）px；HeLa细胞株空白对照组的肿瘤细胞相对迁移距离为（360.667±15.308）px，处理组的相对迁移距离为（13.000±3.606）px。黑种草子总黄酮处理可诱导SiHa和HeLa细胞发生凋亡，SiHa细胞株10、15、25 μg/mL组凋亡率分别为（8.5±0.5）%、（35.9±1.3）%、（85.7±1.6）%；HeLa细胞株10、15、25 μg/mL组凋亡率分别为（5.3±0.4）%、（11.4±0.8）%、（85.9±1.9）%，与空白对照组比较差异均有统计学意义（P < 0.05）。结论：新疆黑种草子总黄酮在体外具有抗宫颈癌细胞增殖和迁移的药理活性，同时能够诱导宫颈癌细胞株发生凋亡。

Toxicological activities of total flavonoids from Xinjiang semen Nigellae in human cervical cancer cell lines

OBJECTIVE:The aim of this study was to investigate the toxicological activityity of total flavonoids from Xinjiang semen Nigellae,the unique national drug resources of Xinjiang,in human cervical cancer cell lines to investigate the mechanism by using. METHODS:Two humna cervical carcinoma cell lines,SiHa and HeLa,were treated with different concentrations of the total flavonoids from the semen nigellae(2.5,5,10,15,20,25,50 μg/mL). Their toxicological activities were detected using the MTT assay which determined the effective concentrations and IC₅₀. Anti-migration effects were detected by cell scratch assay. Apoptosis rates were detected by flow cytometry. RESULTS:Compared with the control group,proliferation of the treated cervical cancer cells was inhibited in a concentration-dependent manner. The IC₅₀ of total flavonoids was 16.935 μg/mL for SiHa and 18.366 μg/mL for HeLa. By comparing the scratch widths before and after the treatments,the relative migration distance of tumor cells in the SiHa cell line control group was (223.333±13.868) px,and that in the treated group was (56.333±10.970) px. The relative migration distance of the HeLa cell control group was (360.667±15.308) px,and that in the treated group was (13.000±3.606) px. Total flavonoids also induced apoptosis in both cervical cancer cell lines. The apoptosis rates in the 10,15 and 25 μg/mL treatment groups were (8.5±0.5)%,(35.9±1.3)% and (85.7±1.6)% for SiHa,and were (5.3±0.4)%,(11.4±0.8)% and (85.9±1.9)% for HeLa. The differences were significantly different from that of the control (P < 0.05). CONCLUSION:The total flavonoids from Xinjiang semen nigellae demonstrated anti-proliferation anti-migration activities in vitro and induced apoptosis in human cervical cancer cell lines.