结直肠癌组织中HMGN5表达及其临床意义

目的探讨结直肠癌组织中高迁移率族核小体结合域5（HMGN5）的水平及其临床意义。方法收集眉山市人民医院2005年4月至2010年10月手术切除的176例结直肠癌组织和78例癌旁正常组织，采用免疫组化检测以上组织中HMGN5的表达水平，比较结直肠癌组织及癌旁正常组织中的HMGN5阳性表达率，分析结直肠癌组织HMGN5阳性表达率与临床病理特征（性别、年龄、肿瘤位置、病理分级、TNM分期和肿瘤大小）的关系，根据随访资料分析不同HMGN5表达水平与预后的关系。结果免疫组化结果显示结直肠癌组织的HMGN5阳性表达率为6875％（121／176），高于癌旁组织的2692％（21／78），差异有统计学意义（P＜005）；结直肠癌组织HMGN5阳性表达率与年龄、性别、肿瘤位置、病理分级和肿瘤大小均无关，与TNM分期有关，其中Ⅲ、Ⅳ期组织的HMGN5阳性表达率为8105％（77／95），高于Ⅰ、Ⅱ期的5432％（44／81），差异有统计学意义（P＜005）。HMGN5阴性表达者的中位生存期为560个月，长于阳性表达者的370个月（P＜005）。结论HMGN5在结直肠癌组织中的表达升高，且阳性表达者的预后较差、TNM分期较晚，可能与结直肠癌的发生发展有关，在结直肠癌诊断及病情评估有一定价值。

#br#Analysis of HMGN5 expression in colorectal cancer tissues and its clinical significance#br#

ObjectiveTo investigate the expression of high mobility group nucleosome binding domain 5（HMGN5） and its clinical significance in colorectal cancer tissues.MethodsOne hundred and seventy six cases of colorectal cancer tissues and 78 cases of adjacent normal tissues were collected in Meishan Peoples Hospital from April 2005 to October 2010. Immunohistochemistry was used to detect the expression of HMGN5 in the above tissues. The positive expression rates of HMGN5 in colorectal cancer tissues and adjacent normal tissues were compared. The relationship between the positive rates of HMGN5 and the clinicopathological parameters of colorectal cancer（sex， age， tumor location， pathological grade， TNM staging and tumor size） was analyzed. Besides， the relationship between HMGN5 expression and prognosis was analyzed based on follow up data.ResultsThe immunohistochemical results showed that the positive expression rate of HMGN5 in colorectal cancer tissues was 6875％（121／176）， higher than 2692％（21／78） of adjacent normal tissues， and the difference was statistically significant（P＜005）. The positive expression rate of HMGN5 in colorectal cancer tissues was not related to age， sex， tumor location， pathological grade and tumor size， but related to TNM staging. The positive expression rate of HMGN5 in tissues of Ⅲ and Ⅳ stage was 8105％（77／95）， higher than 5432％（44／81） of Ⅰ and Ⅱ stage， and the difference was statistically significant（P＜005）. The median overall survival of patients with HMGN5 negative expression was 560 months， longer than 370 months of patients with positive expression（P＜005）.ConclusionThe elevated expression of HMGN5 in colorectal cancer tissues was associated with poor prognosis and advanced TNM stage. It may be related to the development of colorectal cancer， and it has some value in the diagnosis of colorectal cancer and the evaluation of the disease.