2-乙酰基-4-羟基-丁基咪唑对小鼠细胞免疫功能的影响

目的：研究连续暴露2-乙酰基-4-羟基-丁基咪唑（THI）对小鼠细胞免疫功能的影响。方法：选用BALB/c雌鼠作为实验动物，经口连续染毒THI 7 d（染毒剂量分别为0、0.5、2.5、12.5 mg/kg）和30 d（染毒剂量分别为0、0.2、0.5、2.5 mg/kg）。染毒结束后，进行外周血白细胞分类计数、T细胞增殖功能实验和NK细胞活性实验；使用流式细胞术检测胸腺、脾中T细胞数和淋巴细胞亚群变化；使用Transwell小室法检测T细胞趋化功能。结果：与对照组比较，经口连续染毒THI 7 d，2.5和12.5 mg/kg剂量组小鼠外周血白细胞数减少（P < 0.05），CD4⁺和CD8⁺T细胞数增多（P < 0.05），NK细胞活性降低（P < 0.05），T细胞趋化功能受损（P < 0.05）；12.5 mg/kg剂量组外周血淋巴细胞数减少，胸腺T细胞数增多，T细胞增殖功能减弱（P < 0.05）。经口连续染毒THI 30 d，0.5和2.5 mg/kg剂量组小鼠外周血白细胞数减少，胸腺T细胞数增多，T细胞增殖功能减弱，NK细胞活性降低，T细胞趋化功能受损（P < 0.05）。结论：THI可抑制小鼠细胞免疫功能，并引起血液中淋巴细胞减少。该效应具有剂量和时间依赖性。

Effect of 2-acetyl-4-hydroxy-butylimidazole on cellular immune functions in mice

OBJECTIVE:To explore the effects of 2-acetyl-4-hydroxy-butylimidazole(THI) on cellular immune functions in mice. METHODS:BALB/c female mice were exposed to THI for 7 and 30 days. The doses were 0,0.5,2.5,12.5 mg/kg and 0,0.2,0.5,2.5 mg/kg,respectively. After the end of the exposure,peripheral blood leukocyte differential count,T cell proliferation function test and NK cell activity test were performed. Flow cytometry was used to detect T cell number and lymphocyte subset changes in thymus and spleen. Transwell chamber method was used to detect T cell chemotaxis. RESULTS:The results show that after exposure for 7 days,the number of peripheral blood leukocytes was decreased,the numbers of CD4⁺ T cells and CD8⁺ T cells were increased,the NK cell activity was decreased,and the T cell chemotactic function was impaired in the 2.5 and 12.5 mg/kg dose groups. The number of peripheral blood lymphocytes was decreased,the number of thymic T cells was increased,and the T cell proliferation function was decreased in the 12.5 mg/kg dose group. After exposure for 30 days,the number of peripheral blood leukocytes was decreased,the number of thymic T cell was decreased,the T cell proliferation function was decreased,the NK cell activity was decreased and the T cell chemotactic function was impaired in the 0.5 and 2.5 mg/kg dose groups. CONCLUSION:THI inhibited cellular immune function in BALB/c female mice,and caused lymphopenia in the blood. These effects also showed dose and time response relationships.