肉苁蓉苯乙醇总苷脂质体对大鼠肝星状细胞增殖、凋亡及细胞周期的影响

目的：研究肉苁蓉苯乙醇总苷（CPhGs）脂质体对大鼠肝星状细胞（HSCs）增殖、凋亡、周期的影响及其相关分子机制。方法：HSCs分为空白对照组和CPhGs脂质体低、中、高浓度（分别为7.36、14.72、29.45 μg/mL）处理组。采用MTT法检测CPhGs脂质体对大鼠肝星状细胞增殖的影响；流式细胞术Annexin V-FITC/PI双染色法检测细胞凋亡状况，PI染色法检测大鼠肝星状细胞的周期变化；Western blot检测caspase-3、p27蛋白的表达变化。结果：与空白对照组比较，低、中、高3个剂量的CPhGs脂质体作用于HSCs后，均可抑制细胞的增殖（P < 0.05）；3个剂量的CPhGs脂质体均可促使大鼠肝星状细胞发生凋亡，使细胞阻滞在G0/G1期，上调caspase-3及p27蛋白的表达（P < 0.05）。结论：CPhGs脂质体对体外培养大鼠肝星状细胞有抑制增殖、促进凋亡、阻滞细胞周期的作用。其机制可能与CPhGs脂质体上调caspase-3和p27蛋白表达有关。

Effect of Cistanche phenylethanol glycoside liposomes on proliferation,apoptosis and cell cycle of HSC cells in vitro

OBJECTIVE:To investigate the effect of phenylethanol glycoside liposomes from Cistanche (CPhG) on proliferation,apoptosis and cell cycle of HSC-T6. METHODS:HSC cultures were divided into untreated control and treated with low,medium and high concentrations of CPhG liposomes (7.36,14.72,29.45 μg/mL,respectively). Growth inhibition was detected by using the MTT assay,apoptosis was evaluated by Annexin V-FITC/PI staining and cell cycle changes were detected by flow cytometry. Protein expressions of cleaved caspase-3,p27 were detected using Western blotting. RESULTS:Compared with the control group,all treatment doses significantly inhibited cell proliferation and promoted apoptosis (P < 0.05). In addition,cells were arrested in the G0/G1 phase and showed significant up-regulated expression of caspase-3 and p27 proteins (P < 0.05). CONCLUSION:CPhG liposomes inhibited HSC cell proliferation,induced apoptosis and caused cell cycle arrest by up-regulating the protein expression of caspase-3 and p27.