食管鳞癌组织中lncRNA ENST00000589379水平及其对细胞迁移的影响#br#

目的探讨食管鳞癌（ESCC）组织中长链非编码 RNA（lncRNA）ENST00000589379表达及其对ESCC细胞迁移的影响。 方法收集本院2014年1月至2014年12月切除的62例ESCC组织及配对癌旁组织，采用实时定量PCR（QPCR）检测以上组织中的ENST00000589379水平，比较ESCC组织及癌旁正常组织中的ENST00000589379水平，分析ENST00000589379水平与ESCC临床病理参数（年龄、性别、肿瘤大小、分化、T分期、淋巴结转移和TNM分期）的关系；通过在ESCC细胞TE 1 和Eca 109中沉默和过表达ENST00000589379以评估其水平与ESCC细胞转移的关系。 结果QPCR结果显示ENST00000589379在ESCC组织、细胞中的表达水平均高于癌旁组织及正常食管黏膜上皮细胞（P＜005）；ESCC组织中的ENST00000589379水平与年龄、性别、肿瘤大小、分化和T分期无关，但与淋巴结转移和TNM分期有关（P＜005），其中淋巴结转移者的ENST00000589379水平为559±127，高于无淋巴结转移者的420±156，TNM Ⅲ～Ⅳ期的ENST00000589379水平为587±107，高于Ⅰ～Ⅱ期的423±154，差异有统计学意义（P＜005）。Transwell实验发现ENST00000589379沉默可显著降低TE 1、Eca 109细胞迁移，而其过表达可促进TE 1、Eca 109细胞迁移。

Analysis of lncRNA ENST00000589379 level in tissues and its influence on cell migration of esophageal squamous cell carcinoma

ObjectiveTo investigate the level of long non coding RNA（lncRNA） ENST00000589379 in tissues and its influence on cell migration of esophageal squamous cell carcinoma（ESCC）. MethodsSixty two cases of ESCC and paired paracancerous tissues from January 2014 to December 2014 were collected. Using real time quantitative PCR（QPCR）， the level of ENST00000589379 in the above tissues was investigated. The ENST00000589379 levels in ESCC tissues and adjacent normal tissues were compared. The relationship between ENST00000589379 levels and clinicopathological parameters（age， sex， tumor size， differentiation， T stage， lymph node metastasis and TNM stage） of ESCC was analyzed. By silencing and overexpressing ENST00000589379 in ESCC cells TE 1 and Eca 109， the relationship between the expression level and metastasis of ESCC cells was evaluated. ResultsQPCR results showed that the levels of ENST00000589379 in ESCC tissues and cells were higher than those in adjacent tissues and normal esophageal mucosal epithelial cells（P＜005）. The levels of ENST00000589379 in ESCC were not related to age， sex， tumor size， differentiation， and T staging， but related to lymph node metastasis and TNM staging（P＜005）. The ENST00000589379 level of tissues with lymph node metastases was 559±127， higher than 420±156 of those without lymph node metastases， and the level of ENST00000589379 in tissues of TNM Ⅲ Ⅳ stage was 587±107， higher than 423±154 of Ⅰ Ⅱ stage（P＜005）. Silencing ENST00000589379 could obviously reduce the metastasis of TE 1 and Eca 109 cell lines while overpressing ENST00000589379 could promote the metastasis of the two cell lines. ConclusionThe increased level of ENST00000589379 in ESCC， especially in lymph node metastasis and late TNM stage， has a significant effect on the migration of ESCC， and is of certain significance in the prevention and treatment of ESCC.