甲基丙烯酸环氧丙酯的遗传毒性评价

目的：观察甲基丙烯酸环氧丙酯（GMA）诱发体外培养的中国仓鼠肺细胞（V79）微核发生变化情况，对其遗传毒性进行评价。方法：分别采用不同浓度（2.25、4.5、9.0、18.0、36.0 μg/mL）的GMA染毒V79细胞，设置空白对照组和DMSO溶剂对照组，其中染毒3 h处理组分别在加和不加体外活化系统（S9）条件下进行，染毒24 h处理组在不加S9条件下进行。分别计算细胞复制指数和微核细胞率。结果：GMA的浓度在2.25~36.0 μg/mL范围内，无S9的条件下，与空白对照组和DMSO溶剂对照组相比，GMA染毒3和24 h组的V79细胞复制指数均无明显变化，但微核细胞率明显升高，并呈浓度-效应关系；在有S9的条件下染毒3 h，各剂量组的GMA诱发微核细胞率的差异均无统计学意义。结论：在2.25~36.0 μg/mL浓度范围，GMA可致V79细胞的微核率升高，表明GMA可诱发遗传物质损伤，具有遗传毒性。

Genotoxicity evaluation of glycidyl methacrylate

OBJECTIVE: To evaluate genotoxicity of glycidyl methacrylate (GMA) in Chinese hamster lung cells (V79) using the micronucleus assay. METHODS: V79 cells were treated with different doses of GMA (2.25,4.5,9.0,18.0,36.0 μg/mL) for 3 h with or without an in vitro activation system (S9). Controls include a blank and a DMSO group. Another treatment group was treated for 24 h without S9. Cell replication indices and binuclear micronucleus rates were determined. RESULTS: Without S9,the replication index of the treated V79 cells did not change significantly but the incidence of micronuclei in binuclear cells increased significantly in a dose-dependent manner. With the addition of S9,the rate of micronucleated cells in the high-dose group was not statistically significant. CONCLUSION: In the concentration range of 2.25-36.0 μg/mL,GMA induced micronuclei in V79 cells,indicating that GMA is genotoxic.