鼠李糖乳杆菌对头孢曲松引起的幼年期肠道菌群及生长发育异常的改善效果研究

目的探究鼠李糖乳杆菌对头孢曲松引起的幼年期肠道菌群失调、肠管上皮细胞组织及机体损伤的改善效果。方法36只四周龄小鼠随机分为3组,空白组、抗生素组和实验组。实验共计4周:第一周,实验组受试小鼠灌胃头孢曲松及益生菌[鼠李糖乳杆菌GG菌株(Lactobacillus rhamnosus GG)]的菌悬液;抗生素组灌胃头孢曲松;空白组用等量生理盐水。之后各组均停止干预,继续饲养至第四周。提取第一、四周受试小鼠粪便DNA,采用qPCR和二代测序法分析受试小鼠粪便细菌浓度及粪便细菌的种群构成。第四周处死小鼠,使用HE染色观察受试小鼠肠道病理形态。结果第一周空白组受试小鼠体重高于其他两组(P<0.05),停止干预后实验组体重恢复速度快于抗生素组。第四周抗生素组受试小鼠的肝脏指数和回肠肠腺深度低于空白组和实验组(P<0.05)。第四周抗生素组受试小鼠粪便细菌Shannon指数、Simpson指数显著地高于其他两组,实验组的Simpson指数与空白组无统计学差异(P>0.05)。主坐标分析,第一周的实验组和空白组以及第四周的3组群落结果相似度高。而第一周的抗生素组样本与其他组相比群落差异大。结论抗生素可损伤幼年期宿主的肠道菌群的正常构成、妨害宿主肠管上皮细胞组织及机体的正常发育,益生菌有助于改善及修复抗生素对幼年期宿主肠道菌群及机体的有害影响。

Alleviating effects of Lactobacillus rhamnosus against abnormal intestinal microbiota and development induced by ceftriaxone antibiotics in early life

Objective To investigate whether probiotics could alleviate abnormal intestinal microbiota inducedby antibiotics and related body development in early life. Methods 36 mice were randomly divided into threegroups, blank group, antibiotic group and treatment group. The experiment lasted for four weeks. In the first week, themice in the treatment group were given ceftriaxone and Lactobacillus rhamnosus GG, while those in antibiotic groupwere given ceftriaxone only by gavage. The mice in the blank group were given an equal volume of normal saline bygavage. After the intervention of one week, the tested mice were still kept to live to 4 weeks. The mouse fecal DNAin the first and fourth weeks was extracted, and the concentration of fecal bacteria and the profile composition of fecalbacteria were analyzed by qPCR and next-generation sequencing. The mice was sacrificed in the fourth week. HEstaining was used to observe the pathological morphology of intestine tissue of the tested mice. Results In the firstweek, the tested mice in the treatment group and antibiotic group had a significant reduction in body weight comparedwith the blank group (P<0.05). After the intervention, the weight increase of the treatment group mice were faster thanthose of the antibiotic group. In the fourth week, the crypts of ileum and liver index of the antibiotic group were lowerthan that of the other two groups (P<0.05). In the fourth week, Shannon index and Simpson index in the antibioticgroup were higher than those in the other two groups(P<0.05). There was no statistical difference in Simpson indexbetween the treatment group and the blank group(P>0.05). Principal coordinate analysis showed that the fecalmicrobiotia of all tested mice in the fourth week as well as the treatment group mice and the blank group mice in thefirst week could be clustered into one small area. But the fecal microbiotia of the antibiotic group mice in the firstweek were significantly different from other groups. Conclusion The administration of antibiotics might damagethe intestinal microbiota and body development of early life. Probiotics might be helpful to regulate the structure ofintestinal microbiota and could partly repair the damage effect of antibiotics on the