Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid,solid and wide-range protection against viral infection |
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| Affiliation: | 1. Laboratório de Biofísica, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas e da Saúde, Curso de Pós-Graduação em Ciências Veterinárias, IV, UFRRJ, Seropédica, RJ, Brazil;2. Laboratório de Parasitologia, Instituto de Pesquisas Veterinárias Desidério Finamor, Eldorado do Sul, RS, Brazil;3. Laboratório de Quimioterapia Experimental em Parasitologia Veterinária, Departamento de Parasitologia Animal, Instituto de Veterinária, UFRRJ, Seropédica, RJ, Brazil;4. Laboratório de Doenças Parasitárias, Departamento de Parasitologia Animal, Instituto de Veterinária, UFRRJ, Seropédica, RJ, Brazil;5. Departamento de Epidemiologia e Saúde Pública, Instituto de Veterinária, UFRRJ, Seropédica, RJ, Brazil;1. Department of Pharmaceutical Sciences, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA;2. Department of Pharmacy Practice and Science, College of Pharmacy, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA;3. Division of Rheumatology, Department of Internal Medicine, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA |
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| Abstract: | We have recently described the ability of in vitro-transcribed RNAs, mimicking structural domains in the 5′ and 3′ non-coding regions (NCRs) of the foot-and-mouth disease virus (FMDV) genome, to trigger the innate immune response in porcine cultured cells and mice. In this work, the antiviral effect exerted in vivo by these small synthetic non-infectious RNA molecules was analyzed extensively. The susceptibility of transfected newborn Swiss mice to FMDV challenge was tested using a wide range of viral doses. The level of protection depended on the specific RNA inoculated and was dose-dependent. The RNA giving the best protection was the internal ribosome entry site (IRES), followed by the transcripts corresponding to the S fragment. The time course of resistance to FMDV of the RNA-transfected mice was studied. Our results show the efficacy of these RNAs to prevent viral infection as well as to contain ongoing FMDV infection in certain time intervals. Protection proved to be independent of the serotype of FMDV used for challenge. These results support the potential use of the FMDV NCR transcripts as both prophylactic and therapeutic molecules for new FMDV control strategies. |
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