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| FADH2-依赖型卤化酶阳性放线菌1076的筛选、分类鉴定及其代谢产物的结构研究 | |
| 引用本文： | 刘为营,罗敏玉,殷瑜,陈代杰,戈梅,许激扬,钱秀萍.FADH2-依赖型卤化酶阳性放线菌1076的筛选、分类鉴定及其代谢产物的结构研究[J].中国抗生素杂志,2011,36(11). |
| 作者姓名： | 刘为营 罗敏玉 殷瑜 陈代杰 戈梅 许激扬 钱秀萍 |
| 作者单位： | 1. 中国药科大学生命科学与技术学院,南京,210009 2. 上海交通大学药学院,上海200240;上海来益生物药物研究开发中心,上海201203 3. 上海交通大学药学院,上海,200240 |
| 基金项目： | 国家重大新药创制专项“创新药物企业孵化基地建设”资助项目 |
| 摘 要： | 目的 筛选FADH2-卤化酶阳性菌株发现天然卤化物.方法 设计新的简并引物对200株放线菌基因组卤化酶基因扩增以筛选阳性菌株,并进行比对分析；对阳性菌株进行分类鉴定,对其代谢产物进行HPLC-MS分析.结果 筛选得到到51株阳性菌,其中一株与恩拉霉素的同源性为99％.对该编号为1076的菌株进行的分类鉴定结果,确定为Streptomyces macrosporeus.对其代谢产物进行的HPLC-MS分析显示,其代谢产物与恩拉霉素同质.结论 卤化酶阳性菌株1076属于S.macrosporeus的一个菌株,是新的恩拉霉素产生菌. |
| 关 键 词： | 分类鉴定 FADH2-依赖型卤化酶 S.macrosporeus 恩拉霉素 |
Screening, taxonomic identification and metabolite analysis of FADH2-dependent halogenase positive strain 1076 from actinomycetes |
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| Liu Wei-ying,Luo Min-yu,Yin Yu,Chen Dai-jie,Ge Mei,Xu Ji-yang,Qian xiu-ping.Screening, taxonomic identification and metabolite analysis of FADH2-dependent halogenase positive strain 1076 from actinomycetes[J].Chinese Journal of Antibiotics,2011,36(11). | |
| Authors: | Liu Wei-ying Luo Min-yu Yin Yu Chen Dai-jie Ge Mei Xu Ji-yang Qian xiu-ping |
| Institution: | Liu Wei-ying1,Luo Min-yu2,3,Yin Yu2,Chen Dai-jie2,Ge Mei2,Xu Ji-yang1 and Qian Xiu-ping2 (1 School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,2 School of Pharmacy,Shanghai Jiaotong University,Shanghai 200240,3 Shanghai Health Creation Center for Biopharmaceuticals R&D,Shanghai 201203) |
| Abstract: | Objective To screen for FADH2-depedent halogenase positive strains for finding natural halometabolites by mining actinomycetes genome DNA.Methods The novel halogenase gene-speci?c primers,according to ten known FADH2-dependent halogenases,were designed to amplify the conserved regionsin of this protein from 200 actinomycetes strains.Then,the amino acid sequence identities of putative halogenase gene-positive strains were alignmented in the datebases of GenBank.In addition,HPLC-MS method was used to elucidat... |
| Keywords: | Taxonomic identification FADH2-dependent halogenase Streptomyces macrosporeus Enduracidin |
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