| 响应面法优化替考拉宁发酵培养基的研究 |
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| 引用本文： | 邹昆,张奇,夏永.响应面法优化替考拉宁发酵培养基的研究[J].中国抗生素杂志,2011,36(9). |
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| 作者姓名： | 邹昆 张奇 夏永 |
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| 作者单位： | 中国医药集团总公司四川抗菌素工业研究所,成都,610051 |
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| 摘 要： | 目的 通过响应面分析的方法对发酵生产替考拉宁的培养基进行优化,以提高其产量.方法 以一株替考游动放线菌SIIA 04-7-34为试验菌株,采用逐因子实验法确定替考拉宁合成考察因素的参考范围,再采用Plackett-Burman设计法进行培养基的优化.结果 13个实验因子中筛选到3个显著影响因子:葡萄糖、KH2PO4和接种龄.结论 综合评价实验结果表明:优化发酵条件后,替考拉宁发酵产量提高23.3%,实验值与预测值基本相符.
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| 关 键 词： | 替考拉宁 替考游动放线菌 培养优化 响应面分析法(RSA) |
Culture medium optimization for teicoplanin production by response surface analysis |
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| Zou Kun,Zhang Qi,Xia Yong.Culture medium optimization for teicoplanin production by response surface analysis[J].Chinese Journal of Antibiotics,2011,36(9). |
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| Authors: | Zou Kun Zhang Qi Xia Yong |
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| Institution: | Zou Kun,Zhang Qi and Xia Yong(China National Pharmaceutical Group Corporation,Sichuan Industrial Institute of Antibiotics,Chengdu 610051) |
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| Abstract: | Objective Response surface analysis(RSA) was applied to optimize the culture medium for improving the production of teicoplanin.Methods Actinoplanes teichomyceticus SIIA 04-7-34 was starting stain.Single-factor designs were used to determine the ranges of value of each related factor which may effect the production of teicoplanin.Then Plackett-Burman factorial design was carried out,to evaluate the effect of different culture conditions on the production of teicoplanin.Results Thirteen variables were examin... |
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| Keywords: | Teicoplanin Actinoplanes teichomyceticus Culture optimization Response surface analysis |
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