二氢卟酚纳米光敏剂对CAL-27细胞的光动力杀伤效应

目的:研究新合成的超支化聚醚酯-二氢卟酚e6纳米光敏剂[hyperbranched poly(ether-ester)-Chlorin e6 nano-photosensitizer,HPEE-ce6]的体外光动力疗法(photodynamic therapy,PDT)对人舌癌CAL-27细胞(human oral tonguecancer CAL-27 cells)的杀伤作用。方法:合成HPEE-ce6后,通过激光共聚焦显微镜观察CAL-27细胞对HPEE-ce6的内摄作用。以游离二氢卟酚e6(Chlorin e6,ce6)为对照组,CAL-27细胞经激光PDT处理(630 nm连续激光照射,功率密度100 mW/cm2、能量密度12 J/cm2、光照600 s),继续孵育细胞24h后,应用MTT法检测HPEE-ce6对CAL-27细胞的光动力杀伤效率。采用SAS 6.12软件包对数据进行统计学处理。结果:HPEE和ce6共价结合,生成直径为50 nm左右的纳米粒子,HPEE-ce6以胞吞方式进入细胞,主要分布于细胞质内。MTT比色法表明,HPEE-ce6对CAL-27细胞的PDT杀伤作用显著强于ce6(P<0.05)。结论:HPEE-ce6对CAL-27细胞具有良好的光动力杀伤作用,且PDT杀伤效率高于ce6。

In vitro study of the photodynamic therapy of nano-photosensitizer on human oral tongue cancer CAL-27 cells

PURPOSE：To study the effect of photodynamic therapy of HPEE-ce6 nanoparticles on human oral tongue cancer CAL-27 cells.METHODS：Hyperbranched poly（ether-ester）-Chlorin e6 nano-photosensitizer（HPEE-ce6） was prepared by the reaction of HPEE and ce6,and analyzed by confocal laser scanning microscopy（CLSM） for cell internalization.With free chlorin e6（ce6） for the control group,using photodynamic therapy（PDT）,the cells were photo-irradiated with a 630 nm,continuous wave diode laser（fluence rates：100 mW/cm2;fluence：12 J/cm2;irradiation time：600 s）.The cells were incubated for 24 h,then the phototoxicity of HPEE-ce6 nanoparticles on human oral tongue cancer CAL-27 cells was detected by MTT assay.SAS6.12 software package was used for statistical analysis.RESULTS：The nanoparticles with a diameter of 50nm were synthesized by the covalent binding between hyperbranched poly（ether-ester）（HPEE） and ce6.HPEE-ce6 was uptaked by CAL-27 cells in vitro,mainly in cytoplasm.MTT assay showed a significant effect of phototoxicity compared with ce6 control（P0.05）.CONCLUSION：The results indicate that HPEE-ce6 could be used as promising drug carriers in photodynamic therapy for CAL-27 cells.