| 超支化聚醚酯-二氢卟酚e6纳米光敏剂的构建与表征 |
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| 引用本文： | 李平平,周国瑜,沈玲悦,徐青,顾基中. 超支化聚醚酯-二氢卟酚e6纳米光敏剂的构建与表征[J]. 中国口腔颌面外科杂志, 2011, 9(6): 461-466 |
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| 作者姓名： | 李平平 周国瑜 沈玲悦 徐青 顾基中 |
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| 作者单位： | 上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面外科;上海市口腔医学重点实验室,上海200011 |
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| 基金项目： | 上海市重点学科建设项目 |
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| 摘 要： | 目的:研究超支化聚醚酯-二氢卟酚e6纳米光敏剂[hyperbranched poly (ether-ester)-chlorin e6 nanophotosensitizer,HPEE-ce6]的构建过程与表征,及其对人舌鳞癌CAL-27细胞(Tongue cancer CAL-27 cells)体外光动力疗法(Photodynamic therapy,PDT)的杀伤作用.方法:通过一步台成法构建HPEE-ce6纳米光敏剂,用紫外可见吸收光谱和透射电镜观察其表征,通过透射电镜观察CAL-27细胞的形态学改变.结果:HPEE和ce6共价结合,生成直径为50 nm左右的纳米粒子,其治疗区吸收峰比ce6红移了12 nm;经PDT处理后,形态学研究表明,CAL-27细胞发生凋亡或特征性肿胀样坏死.结论:HPEE-ce6可以通过一步法(HPEE和ce6共价交联)合成,并对人舌癌CAL-27细胞具有良好的光动力杀伤作用.
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| 关 键 词： | 光动力疗法 二氢卟酚e6 超支化聚醚酯 药物载体 纳米粒子 |
Construction and characterization of HPEE-ce6 nano-photosensitizer |
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| LI Ping-ping,ZHOU Guo-yu,SHEN Ling-yue,XU Qing,GU Ji-zhong. Construction and characterization of HPEE-ce6 nano-photosensitizer[J]. China Journal of Oral and Maxillofacial Surgery, 2011, 9(6): 461-466 |
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| Authors: | LI Ping-ping ZHOU Guo-yu SHEN Ling-yue XU Qing GU Ji-zhong |
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| Affiliation: | LI Ping-ping,ZHOU Guo-yu,SHEN Ling-yue,XU Qing,GU Ji-zhong.(Department of Oral and Maxillofacial Surgery,Ninth People's Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Stomatology.Shanghai 200011,China) |
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| Abstract: | PURPOSE: To construct and study the characteristics of hyperbranched poly(ether-ester)-chlorin e6 nano-photosensitizer(HPEE-ce6);and the cytocidal effect of HPEE-ce6 on tongue cancer CAL-27 cells.METHODS: HPEE-ce6 nanoparticles were prepared by reaction of hyperbranched poly(ether-ester)(HPEE) and chlorin e6(ce6) by one-step method,and characterized by ultraviolet-visible absorption spectroscopy(UV-Vis) and transmission electron microscopy(TEM).The morphological changes of the CAL-27 cells were observed by ... |
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| Keywords: | Photodynamic therapy Chlorin e6 Hyperbranched poly(ether-ester) Drug delivery system Nanoparticles |
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