| 工业羽毛蛋白胨对红霉素基因工程菌ZL1004发酵过程的影响 |
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| 引用本文： | 陈竹,李万钧,储炬,庄英萍,张嗣良. 工业羽毛蛋白胨对红霉素基因工程菌ZL1004发酵过程的影响[J]. 中国抗生素杂志, 2011, 36(10) |
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| 作者姓名： | 陈竹 李万钧 储炬 庄英萍 张嗣良 |
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| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室,上海,200237
2. 宜都东阳光生化制药有限公司,湖北,443300 |
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| 基金项目： | 国家高新技术研究计划(863重点项目)，国家科技支撑项目 |
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| 摘 要： | 本文在50L发酵罐上研究了工业羽毛蛋白胨对红霉素基因工程菌ZL 1004发酵过程的影响,结果表明发酵培养基中加入较多的羽毛蛋白胨不利于红霉素的合成,而加入适量的羽毛蛋白胨可有效提升整个发酵过程的摄氧率(OUR)水平、促进菌体对碳氮源的利用和红霉素的合成.进一步对培养基进行优化,采用0.6%羽毛蛋白胨、3.3%黄豆饼粉,发酵结束时红霉素效价达12487U/mL,A组分达9774μg/mL,分别比对照高18.21%和19.94%.利用显微图像及形态分析软件对发酵周期内的菌丝形态进行了跟踪定量研究,分析表明培养基中加入适量羽毛蛋白胨可优化菌丝形态,使其朝有利于红霉素合成的方向进行分化.
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| 关 键 词： | 红霉素 工业羽毛蛋白胨 基因工程菌 菌丝形态 |
The effects of industrial feather peptone on the erythromycin fermentation process by recombinant industrial strain Saccharopolyspora erythraea ZL1004 |
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| Chen Zhu,Li Wan-jun,Chu Ju,Zhuang Ying-ping,Zhang Si-liang. The effects of industrial feather peptone on the erythromycin fermentation process by recombinant industrial strain Saccharopolyspora erythraea ZL1004[J]. Chinese Journal of Antibiotics, 2011, 36(10) |
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| Authors: | Chen Zhu Li Wan-jun Chu Ju Zhuang Ying-ping Zhang Si-liang |
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| Affiliation: | Chen Zhu1,Li Wan-jun2,Chu Ju1,Zhuang Ying-ping1 and Zhang Si-liang1(1 State Key Laboratory of Bioreactor Engineering ECUST,Shanghai 200237,2 Yidu HEC Biochem.Co.Ltd,Hubei 443300) |
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| Abstract: | The effects of industrial feather peptone(IFP) on the erythromycin fermentation process of recombinant strain ZL1004 were investigated in 50L fermenter.The results showed that adding too much IFP was not beneficial for the erythromycin synthesis,while adding appropriate amount of IFP could effectively enhance the OUR level and improve substrate utilization as well as erythromycin production.The fermentation medium was further optimized with 3.3% soybean meal and 0.6% IFP,by the end of fermentation the eryth... |
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| Keywords: | Erythromycin Industrial feather peptone Recombinant strain Morphology |
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