蚓激酶对大鼠缺血后肢腓肠肌的影响及其机制

目的 探讨不同时段蚓激酶对于大鼠缺血后肢腓肠肌的影响及机制.方法 成年Wistar大鼠150只,显微外科技术制作大鼠后肢缺血模型,随机分为蚓激酶治疗组、非治疗组及空白对照组.治疗组从术后1 d开始,于大腿肌肉分4点等量注射蚓激酶(5 g/L,1.6ml/kg体重)每周3次,治疗2周;非治疗组在同样部位按1.6 ml/kg体重注射生理盐水,空白组仅切开皮肤后缝合.分别于术后3、7、14、21、28 d采取缺血后肢及对侧的腓肠肌.测定各组肌肉组织含水量、线粒体游离钙浓度和线粒体通透性转运通道开放程度(用吸光度变化AS表示)的动态变化.结果 缺血腓肠肌含水程度在1周内升高,随后下降,蚓激酶治疗组腓肠肌含水程度在造模术21、28 d后显著高于非治疗组(P＜0.05);在术后第14、21天,蚓激酶治疗组线粒体游离钙浓度显著低于非治疗组(P＜0.05);蚓激酶治疗组中作为MPTP开放程度指标的AS值在第14、21、28天均显著高于非治疗组(P＜0.05).结论 蚓激酶能维持大鼠缺血后肢腓肠肌的含水量,降低缺血腓肠肌内线粒体游离钙浓度,抑制MPTP的开放,表明蚓激酶对大鼠后肢缺血有治疗作用.

The effect of lumbokinase on gastroenemius in the ischemic hindlimbs of rats

Objective To investigate the effect and the mechanism of lumbrokinase on the gastroenemius in the ischemic hindlimbs of rats. Methods One hundred and fifty adult Wistar rats were divided into lumbrokinase-treated group,non-treated group and control group randomly, after establishing the hindlimb ischemic model by microsurgery. The rats were sacrificed at 3rd,Tth, 14th,21 st and 28th day after the operation respectively. The gastreenemius in the ischemic hindlimbs and the opposite normal hindlimbs were harvested for the further research. The water content,the free calcium concentration of the mitochondria, the opening of mitochondria permeability transition pore (MPTP) in the ischemic gastroenemius from the 3 groups were detected. Results The water content of the gastroenemius in the lumbrokinase-treated group was significantly higher than that in non-treated group at 21st and 28th day after ischemia (P ＜ 0.05). The free calcium concentrations in the mitochondria were significantly lower in treated group at 14th and 21st day than in the non-treated group after ischemia ( P ＜ 0.05 ). The opening of MPTP ( expressed by AS) of the gastroenemius in the treated group was significantly higher than that in the non-treated group at 14th ,21 st and 28th day after ischemia ( P ＜ 0.05 ). Conclusion Lumbrokinase could decline the loss of water content in the later period of ischemia,limit the concentration of miteehondrial free calcium and inhibit the opening of the MPTP in the ischemic gastroenemius of rats, which suggests that lumbokinase is effective in the treatment of the ischemia hindlimb muscles of rats.