鼠感染Babesia microti和 Babesia rodhaini后早期 IL-12、IL-10诱生的研究

目的 研究Babesia microti 和Babesia rodhaini感染后早期IL-12和IL-10诱生情况,以探讨B. microti和 B. rodhaini不同种属感染的发病机制与免疫应答效应.方法 使用ELISA方法分别检测Babesia microti 和 Babesia rodhaini 感染鼠后的0、3、6、9、12、18、24、36、48、72、96 h血清中IL-12及IL-10的浓度.结果 B. microti感染后3 h,小鼠血液中IL-12产生达一个高峰,至感染后24 h达最高峰值,与对照组比较差异显著,同组鼠在感染的早期(96 h前)血液中IL-10水平无明显变化.而B. rodhaini感染鼠,在感染后3～72 h,血清中IL-10和IL-12水平与对照组比较均无明显差异.在感染后96 h,血清中IL-10和IL-12水平开始下降,与对照组比较差异显著.结论 B. microti 感染鼠早期诱生的细胞因子主要为IL-12,而B. rodhaini感染后早期IL-12和IL-10水平无明显变化.

Early production of IL-12 and IL-10 in mice infected with Babesia microti and Babesia rodhaini

Objective To examine the serum levels of IL-12 and IL-10 in mice infected with Babesia microti and Babesia rodimiui. Methods The mouse serum was collected and the concentration of IL- 12 and ID 10 was examined using EIJSA after infecting with Babesia microti and Babesia vodhaini at 0, 3, 6, 9, 12, 18, 24, 36, 72, 96 h. Results Babesia microti infection resulted in increasing IL-12, which reached the peaks at 3 and 24 h after the infection, while this infection did not induce a significant change in IL- 10 when compared to uninfected control mice. In Babesia vodhaini-infected mice, no any significant changes in both IL-12 and IL-10 were detected compared with the uninfected control animals during the period of 3 - 72 h after infection, but a significant decline in IL- 12 and IL- 10 was detected at 96 h after infection compared with uninfected control mice. Conclusion The mutagenetic cytokine is IL-12 in the serum of mice after infection with Babsia microti. Babesia rodhaini infection can not induce any significant changes in both IL- 12 and IL- 10 from 0 to 72 h.