用连续培养变形链球菌的方法产生釉质初期龋的研究

用连续培养变形链球菌(简称“变链菌”)的技术在37个离体双尖牙上产生釉质脱矿。用扫描电子显微镜观察了“变链菌”在釉质表面附着和菌斑形成情况,用显微射线照像、透射光和编光显微镜观察到了牙磨片上初期釉质龋损。这个研究建立了一种产生釉质类龋损的方法,以便进行龋齿病因学和防龋研究。

A Study on Serial Culture of Streptococcus Mutans—induced Early Enamel Caries

Demineralisation of enamel on sound human premolars was produced by Streptococcus mutans using a sequential culture technique. Adhesion of Streptococcus mutans on emamel surface and formation of plaque were observed by scanning electronic microscope. Initial enamel lesion on sections were detected in microradiography under transmitted and polarized light.This study established a method for the production of caries-like lesion,so that the aetiology of dental caries and the prevention of caries can be studied.