糖尿病兔视网膜bFGF的表达及定位

目的：探讨糖尿病兔视网膜碱性成纤维生长因子(bFGF)的表达时期及bFGF表达增加与视网膜 Müller细胞的相关性。 方法：16只大耳白兔随机分为正常对照组及糖尿病组，建立糖尿病兔模型，饲养7周后，取材并通过激光共聚焦显微镜应用免疫荧光双重标记方法观察视网膜，对bFGF进行定位。 结果：正常对照组兔眼视网膜各层结构清晰，细胞排列紧密、整齐，细胞形态正常，GFAP免疫荧光标记，显现红色荧光的部位为兔视网膜Müller细胞。bFGF免疫荧光标记仅在毛细血管基底膜处呈现红色荧光；糖尿病组兔视网膜各层结构清晰，内核层、神经节细胞层细胞排列疏松，细胞间隙增大，细胞形态与正常对照组无明显差异，可见出血未见血管新生，bFGF 免疫荧光标记，视网膜各层细胞均见不同强度的红色荧光，GFAP及bFGF双重免疫荧光标记，视网膜可见黄色荧光。糖尿病兔模型的视网膜病变为BDR期；bFGF在BDR期表达增加；bFGF与GFAP共同表达于视网膜Müller细胞。 结论：bFGF在BDR期即开始在视网膜Müller细胞中表达。

Expression and position fixing of bFGF in retina of diabetic rabbits

Objective To investigate the expression period of bFGF and the correlation between the Müller cells in retina and the increasing of bFGF expression in diabetic rabbits.Methods Sixteen rabbits were divided randomly into normal control and diabetic groups.After the models of diabetic rabbits were set up,the rabbits were breeded for 7 weeks.The immunofluorescence double labeling method and laser confocal microscopy were used to observe the ratina of the diabetic rabbits and the position fixing of bFGF was studied.Results In normal control group,the structure of each layer of retina was clear,the cell lined up tightly and regularly with normal shape,the location with red fluorescence was Müller cells signaled with GFAP,labeled with bFGF,only on capillary basement membrane the red fluorescence could be found.In diabetic group,the structure of each layer of retina was clear,the cells of INL and GCL lined up loosely,the intercellular space increased,there was no significant difference compared with normal group on cell shape.Labeled with bFGF,the different intensity red fluorescence could be found in each layer cells,double labeled with GFAP and bFGF,the yellow fluorescence could be found.The retinopathy of diabetic rabbit was in BDR stage.The expression of bFGF increased in this stage.The bFGF and GFAP coexpressed in Müller cells.Conclusion The bFGF begins to express in the Müller cells from the BDR stage.