硒化壳聚糖通过下调PML-RARα融合蛋白来对NB4细胞产生抑制增殖和诱导凋亡作用

目的 研究硒化壳聚糖对急性早幼粒白血病细胞株NB4增殖和凋亡的影响,探讨这种影响与PML-RARα融合蛋白表达的关系.方法 应用MTT法检测硒化壳聚糖对细胞增殖的影响,应用AO/EB荧光染色法共聚焦显微镜下观察诱导凋亡作用,应用Western blot的方法检测细胞PML-RARα融合蛋白含量的变化.结果 硒化壳聚糖可对体外培养的NB4细胞产生剂量和时间依赖性的抑制作用,50 mg/L 至 100 mg/L硒化壳聚糖作用NB4细胞24 h可诱导细胞出现明显的凋亡现象,经硒化壳聚糖处理24 h后,NB4细胞内PML-RARα融合蛋白含量明显减少.结论 硒化壳聚糖可通过下调PML-RARα融合蛋白含量对NB4细胞产生抑制增殖和诱导凋亡作用.

Effect of Selenium Chiston on Proliferation and Apoptosis of NB4 Cells

Objective To investigate the effects of Selenium chiston (Sc) on proliferation and apoptosis of NB4 cells and explore its relationship with PML-RARα fusion proteins.Methods MTT was used to determine the proliferative effects of drugs on NB4 cells and AO/EB fluoresent staining method was used to observe the inducing apoptosis effect of drugs. Western blot was used to examine the abundance of PML-RARα fusion proteins expressed by tumor cells.Results An exposure of NB4 cells to Sc produced both concentration and time-dependent increase in the anti-proliferative rate.Treatment with 50 mg/L to 100 mg/L of Sc for 24 h could induce NB4 cells apoptosis. The abundance of PML-RARα fusion proteins was strongly down-regulated in Sc-treated NB4 cells.Conclusion Selenium chiston can inhibit the proliferation and induce apoptosis of NB4 cells and these effects are correlated with down-regulation of the abundance of PML-RARα fusion proteins.