Effects of CD11/CD18 mediated PMN-EC adhesion on the endothelial cell damages in the early stage postburn in vitro

Activatedpolymorphonuclearneutrophils(PMNs)havebeenshowntoproducedamagesonvascularendothelialcells(ECs)throughthegener-ationofoxygen-derivedfreeradicalsandthere-leaseofproteases;andPMN-ECadhesionisessen-tialtomediateECdamages'j.TheintegrinCD11/CD18consistsof3heterodimericsubunits,eachofwhichcomprisesadistinctAchain(CDl1a,CDl1bandCD1lc)thatisnoncovalentlyassociatedwitlhacommonBchain(CD18).TheimportanceoftheCD1l/CD18integrininPMNadhesionandemigrationinsystemicvasculaturehasbeencom-…

Effects of CD11/CD18 mediated PMN-EC adhesion on the endothelial cell damages in the early stage postburn in vitro

Objective: To investigate the effects of CD11/CD18 on the endothelial cell (EC)damages induced by polymorphonuclear neutrophils (PMN )from patients in the early stage postburn. Metbods: PMNs from 10 patients in the first 24 to 48 h postburn were incubated with cultured human umbilical vein ECs (HUVECs) for 24 h and the percentage of PMN-EC adhesion was calculated, the content of lactate dehydrogenase (LDH), angiotensin converting enzyme (ACE) and 6-keto-PGF,. in the medium determined and the morphological changes Qf ECs observed. Results: After incubation with PMNs from burn victims, deformation of the HUVECs cell shrinkage, and formation of intercellurar space occurred and some cells were detached from the vascular wall. The content of LDH, ACE and 6-keto-PGF,. increased. When the PMNs were pretreated with monoclonal antibodies (McAbs)against CD11a/CD11b or CDl1b/CD18, the PMN-EC adhesion rate and the content of LDH, ACE and 6-ketoPGF,. in the culture medium decreased and Ec damages attenuated especially when the 2 kinds of McAbs were used in combination. Conclusion: The findings indicate that in the early stage postburn, PMNs can induce damages of ECs which results from CD1l/CD18 mediated PMN-CE adhesion and that the administration of McAbs against CD11/CD18 mediated PMN-EC adhesion and that the administration of McAbs against CD11/CD18 is able to b1ock partially the PMN-EC adhesion in burn patients and attenuate the damage of ECs.