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Micromethod for determination of ceftriaxone in plasma and urine by high-performance liquid chromatography
Authors:Demotes-Mainard F M  Vinçon G A  Jarry C H  Albin H C
Affiliation:1. Equine Clinic, University of Veterinary Medicine Hanover, Foundation, Hanover, Germany;2. Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hanover, Foundation, Hanover, Germany;3. Institute of Biometry and Information Processing, University of Veterinary Medicine Hanover, Hanover, Germany;4. Center for Systems Neuroscience Hanover, University of Veterinary Medicine Hanover, Hanover, Germany;1. Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB21QT, UK;2. Department of Neuroscience, Erasmus MC, NL-3015 GE Rotterdam, The Netherlands;1. Department of Biochemistry, School of Medicine, Ardabil University of Medical University, Ardabil, Iran;2. Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran;3. Department of Midwifery, School of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran;4. Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran;5. Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran;1. Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil;2. Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada;3. Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, United Kingdom;4. Seroepidemiology, Cellular and Molecular Immunology Laboratory - Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil;1. Department of Medicine, Hammersmith Hospital, Imperial College London, UK;2. Breakspear Medical Group, Hemel Hempstead, Hertfordshire, UK;1. Dipartimento di Farmacia, Orali e Biotecnologiche – Università degli Studi “G. D’Annunzio” Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy;2. Dipartimento di Scienze Mediche, Orali e Biotecnologiche – Università degli Studi “G. D’Annunzio” Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy;3. Dipartimento di Medicina Clinica, Sanità Pubblica, Scienze della Vita e dell’Ambiente – Università degli studi di L’Aquila – 671100, L’aquila, Italy
Abstract:A sensitive and rapid high-performance liquid chromatographic method for the determination of ceftriaxone in human plasma and urine is described. A C18 reversed phase column is used; the mobile phase comprises water-methanol-triethylamine (750:250:4v/v/v) adjusted to pH 3 with orthophosphoric acid. Quantitation is performed at 270 nm with cefazolin as the internal standard. This method involves precipitation of proteins from fluids with acetonitrile followed by extraction of endogenous compounds with chloroform and injection of the upper aqueous phase on to the chromatograph. Relative standard deviations for between-day and within-day assays are 6.2%. The detection limit is 0.5 mug(-1) in plasma and urine. Studies of drug stability during sample storage, sample pretreatment and chromatography showed no degradation of ceftriaxone or of the internal standard. The method is convenient for clinical monitoring and for pharmacokinetic studies.
Keywords:
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