荧光定量聚合酶链反应检测对呼吸道感染患儿EB病毒的诊断价值

目的探讨荧光定量聚合酶链反应（PCR）检测对呼吸道感染患儿EB病毒DNA（EBV—DNA）的诊断价值。方法采用荧光定量PCR技术（FQ—PCR）检测127例呼吸道感染患儿呼吸道分泌物标本中EBV—DNA。结果EBV—DNA阳性68例（53．5％），其中-1岁12例，-3岁23例，-5岁18例，-7岁10例，-9岁3例（，～13岁2例。外周血白细胞计数〉10。0X10。／L21例，自细胞计数〈10．0X10。／L8例，单核细胞计数〉10％9例，异淋巴细胞〉10％10例，血小板减少症4例，肝功能异常5例，心肌酶异常8例。结论采用荧光定量PCR技术检测呼吸道感染患儿EB-DNA有较高的临床诊断价值。

The diagnostic value of fluorescence quantitative polymerase chain reaction for the detection of respiratory tract infection of children with EB virus

Objective To investigate the fluorescence quantitative polymerase chain reaction and teat its diagnostic value of DNA(EBV-DNA)of EB virus in respiratory tract infection in children.Methods To adopt fluorescent quantitative PCR technology(FQ-PCR)to examine EBV-DNA in the aspirate specimen from 127 cases of children.Results 68 cases of EBV-DNA positive(53.5％),including 1year of age:12;3 years of age:23;5 years of age:18;7 years of age:10;9 years of age:3 and 13 years of age:2.The number of peripheral white blood cells ＞ 10.0× 109/L:21 cases;the number of white blood cells ＜ 10.0 × 109/L:8 cases;the number of mononuclear cells ＞ 10％:9 cases;abnormal lymphocyte ＞ 10％:10 cases;thrombocytopenia:4 cases;abnormal liver fumction:5 cases; myocardial enzyme abnormalities:8 cases.Conclusion The fluorescent quantitative PCR technology to test EB-DBA of respiratory tract infection in children had hight diagnostic value.