| HPLC法测定蛤蚧中胆甾醇含量 |
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| 引用本文： | 邱葵,吴华,王鹤尧.HPLC法测定蛤蚧中胆甾醇含量[J].中国中医药信息杂志,2008,15(9):43-44. |
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| 作者姓名： | 邱葵 吴华 王鹤尧 |
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| 作者单位： | 首都医科大学附属北京朝阳医院,北京,100020 |
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| 摘 要： | 目的建立高效液相色谱法测定蛤蚧中胆甾醇的含量。方法采用高效液相色谱法，色谱柱：Hypersil ODS2柱（4．6mm×150mm，5m）；流动相：甲醇；检测波长208nm；柱温40℃；流速为1．0mL／min。结果胆甾醇质量在0．885～14．000μg范围与峰面积值的线性关系良好（r=0．9995），平均回收率99．65％，RSD=3．45％（n=6）。结论本方法简便可行、快速准确，可用于蛤蚧中胆甾醇的含量测定。
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| 关 键 词： | 蛤蚧 胆甾醇 高效液相色谱法 含量测定 |
High Performance Liquid Chromatography Method for the Quantification of Cholesterol in Gekko Gecko |
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| QIU Kui,WU Hua,WANG He-yao.High Performance Liquid Chromatography Method for the Quantification of Cholesterol in Gekko Gecko[J].Chinese Journal of Information on Traditional Chinese Medicine,2008,15(9):43-44. |
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| Authors: | QIU Kui WU Hua WANG He-yao |
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| Institution: | QIU Kui,WU Hua,WANG He-yao (Beijing Chaoyang Hospital Affiliated to Capital University of Medical Sciences,Beijing 100020,China) |
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| Abstract: | Objective To determine the content of cholesterol in Gekko Gecko. Methods High performance liquid chromatography was performed on Hypersil ODS2 （4.6 mm× 150 ram, 5 μm）. The chromatorgraphic conditions were as follows： methanol as mobile phase, flow rate being 1.0 mL/min and detecting wavelength at 208 nm. The column temperature was 40 X2. Results A good linearity of cholesterol was shown in range of 0.885-14.000 μg （r=0.999 5）, the average recovery was 99.65%, RSD=3.45% （n=6）. Conclusions This method is simple, rapid and accurate, and may provide a reliable way for the quantification of cholesterol in Gekko Gecko. |
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| Keywords: | Gekko Gecko cholesterol HPLC content determination |
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