短柄小连翘中乙酸乙酯部位化学成分的研究

采用乙醇提取和液液萃取的方法对短柄小连翘茎枝进行了提取.通过硅胶柱色谱、Sephadex LH-20柱色谱、反相中压柱色谱和高效液相等多种色谱方法相结合,从短柄小连翘乙醇提取物的乙酸乙酯部位中分离得到了7个双环氧木脂素化合物.经MS和NMR等波谱技术分别鉴定为松脂醇(pinoresinol,1),5'-甲氧基-松脂素(medioresinol,2),8-乙酰基松脂醇(8-acetoxypinoresinol,3),表松脂醇(epipinoresinol,4),(+)-丁香树脂醇(+)-syringaresinol(5)]、(+)-1-羟基丁香树脂醇(+)-1-hydroxysyringaresinol(6)]和erythro-buddlenolE(7).化合物 1~7 均为首次从该植物中分离得到.抗氧化活性筛选结果显示,化合物7在1×10^-6 mol·L^-1浓度下,对Fe^2+_Cys诱导大鼠肝微粒体丙二醛(MDA)生成的抑制率为38%,而相同浓度下,阳性对照药Vit E的抑制率为35%,表明化合物7具有显著的抗氧化作用.

Ethyl acetate-soluble chemical constituents from branch of Hypericum petiolulatum

The stems and branches of Hypericum petiolulatum were extracted by alcohol and liquid-liquid extraction. Seven furofuranlignans were isolated from the ethyl acetate fraction of ethanol extract of H. petiolulatum by using silica gelchromatography, Sephadex LH-20 chromatography, medium-pressure liquid chromatography and preparative HPLC. Their structures were identified by the spectroscopic methods as pinoresinol(1), medioresinol(2), 8-acetoxypinoresinol(3), epipinoresinol(4),(+)-syringaresinol(5),(+)-1-hydroxysyringaresinol(6) and erythro-buddlenolE(7).All the isolates were firstly found in H. petiolulatum.In the bioassay, compound 7 showed remarkable antioxidative activity inhibiting Fe⁺²-cystine induced rat liver microsomal lipid peroxidation with inhibitory rate 38%at a concentration of 1×10^-6 mol·L^-1(positive control Vit E with the inhibitory rate of 35% at the same concentration).