黎药裸花紫珠在小鼠体内止血活性部位的研究

目的 确定黎药裸花紫珠的止血活性部位,为其止血活性成分的研究提供依据。方法 裸花紫珠经醇提获得醇提物(EtOH-extract)、醇提物经大孔吸附树脂处理分别获得水洗脱物(HP-H2O-elution)和乙醇洗脱物(HP-EtOH-elution),将EtOH-extract、HP-EtOH-elution和HP-H2O-elution分别配制成高、中、低剂量的供试药物,云南白药为阳性对照。每日给小鼠灌胃1次,在连续给药7d后,记录小鼠体质量的变化,采用断尾法和玻片法测定小鼠的出血时间和凝血时间,并以此为筛选指标对各部位止血效果进行评价。结果 与模型对照组相比,HP-EtOH-elution对缩短小鼠出血时间的作用效果显著,并且其低剂量组和中剂量组的效果均优于阳性对照组。EtOH-extract、HP-EtOH-elution和HP-H2O-elution在缩短小鼠的凝血时间和增加小鼠的体质量方面,效果均不显著。结论 裸花紫珠的止血活性成分主要集中在醇提物经大孔树脂处理后的乙醇洗脱部位,并且可能是通过影响内源性凝血途径发挥止血作用。

Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo

Objective To screen the hemostatic active extracts from Callicarpa nudiflora.Methods The powdered Callicarpa nudiflora was extracted with 70% EtOH and concentrated to give EtOH-extract. The EtOH-extract was further chromatographed over HP-20 macroporous resin column, eluting with aqua and 95% EtOH to get HP-H₂O-elution and HP-EtOH-elution, respectively. The obtained three extracts of EtOH-extrract, HP-EtOH-elution and HP-H₂O-elution were set as large, middle and small dosage groups for drug preparation, respectively. Yunnan Baiyao was used as a positive control group.The weight increment, bleeding time and clotting time of fed mice were detected by cutting tail and grass slide Methods after intragastric administration for 7 days.Results As compared with blank model and positive control, each dosage groups of HP-EtOH-elution could significantly shortened the bleeding time, of which the small dosage group and middle dosage group even exhibited better Results than the positive control group.Whereas the EtOH-extract, HP-EtOH-elution and HP-H₂O-elution didn't demonstrated significant effect on clotting time as well as the weight increment. Conclusion The HP-EtOH-elution was suggested to be the major active extract possessing hemostatic activity to mice under tested dosages.The hemostatic mechanism may through intrinsic coagulation pathway.This study would be helpful for further phytochemical investigation of C.nudiflora.