磷酸化Rb在苯并[a]芘致皮质神经元凋亡中的作用

目的探讨磷酸化视网膜母细胞瘤蛋白（phospho-retinoblastoma protein，p-Rb）在苯并[a]芘致神经元凋亡过程中的作用。方法用CCK-8试剂检测细胞活性；Annexin—V／PI双染法检测细胞凋亡率，免疫荧光细胞化学染色观察p．Rb表达，免疫印记法（Western-blot）检测p—Rb的表达情况。结果与对照组相比，各染毒组神经元活性降低（P〈0．05），凋亡率增高（P〈0．05），p-Rb表达量增高（P〈0．05），2μmol／L组加入抑制剂后细胞活性增强（P〈0．05），凋亡率下降（P〈0．05），p—Rb阳性细胞数减少（P〈0．05），p-Rb表达量降低（P〈0．05）。结论苯并[a]芘可引起磷酸化Rb水平升高，是苯并[a]芘致神经元凋亡的可能机制。

Role of phospho-Rb in cortical neurons apoptosis induced by benzo[ a ] pyrene

[ Objective]To explore the role of phospho-Rb （p-Rb） in cortical neurons apoptosis induced by benzol [a]pyrene （B[a]P）. [Methods] The cell viability was determined by Cell Counting Kit 8. Cell apoptotic rates were detected by Annexin-V/PI assay. Immunofluorescence staining was used to observe the expression of p-Rb. The expression of p-Rb was detected by western-blot. [ Results] Compared with the control group, the neuronal viability in poisoned groups were decreased （P 〈 0.05}, apoptotic rates were increased （ P 〈 0.05 ）, the expressions of p-Rb in each group were increased （ P 〈 0. 05 ）. The 2 μmol/L group with the addition of inhibitive showed the enhanced cell viability （ P 〈 0. 05 ） , decreased apoptotic rates （ P 〈 0. 05 ） , decreased number of p-Rb positive cells （ P 〈 0. 05 ）, and the decreased expressions of p-Rb （ P 〈 0.05 ）. [ Conclusion] B [ a ] P can induce the rising of expression level of p-Rb, which may be a probable mechanism of neuron apoptosis induced by B[ a] P.