| 长期慢性束缚水浸应激对大鼠胃窦Cajal间质细胞数量的影响 |
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| 引用本文： | 张丹,夏志伟,韩亚京. 长期慢性束缚水浸应激对大鼠胃窦Cajal间质细胞数量的影响[J]. 世界华人消化杂志, 2010, 18(9) |
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| 作者姓名： | 张丹 夏志伟 韩亚京 |
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| 作者单位： | 北京大学第三医院消化科,北京市,100191;北京大学第三医院消化科,北京市,100191;北京大学第三医院消化科,北京市,100191 |
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| 摘 要： | 目的:研究不同时程低强度束缚水浸应激对大鼠Cajal间质细胞(interstitial cells of Cajal,ICC)数量的影响,探讨慢性应激导致胃动力改变与ICC的相关性.方法:雄性SPF级SD大鼠48只被随机分为6组,即实验3 d、7 d、28 d组和对照3 d、7 d、28 d组,每组8只.实验组23℃水域箱内束缚水浸1h/d,对照组自由摄食饮水.4 d、8d、29d晨序贯脱颈处死.取膜胃小弯侧(ICC-1)、腺胃小弯侧(ICC-3)、腺胃大弯侧上1/3处(ICC-5)、腺胃幽门旁0.5 cm处(ICC-7)组织各一块放入固定液中,制成石蜡切片:抗c-kit免疫组织化学染色,观察不同层次ICC计数.结果:正常大鼠胃内ICC主要分布在肌内(ICC-MY)和肌间(ICC-IM),而黏膜下(ICC-SM)和深肌丛(ICC-DMP)较少.实验3 d组的各层ICC数量与对照组相比无明显差异,实验7 d和28 d组大鼠不同部位、不同层次及同一部位不同层次ICC数量明显异于同期对照组.应激时间长短对不同部位不同层次及同一部位不同层次ICC总数有明显影响,其大致规律是先正常后增加再减少,即先升高后降低.应激主要引起ICC-MY及ICC-IM数量发生变化,而本来较少的ICC-SM和ICC-DMP受影响较轻.结论:长时间低强度的慢性束缚水浸应激可以使大鼠胃窦ICC数量发生变化.
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| 关 键 词： | 胃 Cajal间质细胞 慢性束缚水浸应激 |
Long-term chronic water immersion-restraint stress alters the number of interstitial cells of Cajal in the gastric antrum of rats |
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| Dan Zhang,Zhi-Wei Xia,Ya-Jing Han. Long-term chronic water immersion-restraint stress alters the number of interstitial cells of Cajal in the gastric antrum of rats[J]. World Chinese Journal of Digestology, 2010, 18(9) |
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| Authors: | Dan Zhang Zhi-Wei Xia Ya-Jing Han |
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| Affiliation: | Dan Zhang,Zhi-Wei Xia,Ya-Jing Han,Department of Digestive Diseases,Peking University Third Hospital,Beijing 100191,China |
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| Abstract: | AIM: To investigate the impact of long-term chronic water immersion-restraint stress on the number of interstitial cells of Cajal (ICC) in the gastric antrum of rats and to explore the relationship between ICC and gastric motility changes caused by chronic stress. METHODS: Forty-eight male Sprague-Dawley rats were randomly and equally divided into six groups: three experiment groups and three matched control groups. The three experimental groups underwent water immersion-restraint stress for one hour daily ... |
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| Keywords: | Stomach Interstitial cell of Cajal Chronic water immersion-restraint stress |
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