灰树花提取物对脾虚小鼠腹腔巨噬细胞和脾细胞的影响

目的:探讨灰树花提取物对脾虚小鼠的免疫调节机制。方法:健康雄性清洁级昆明小鼠30只随机分为6组,即空白对照组、脾虚组、阳性对照组(香菇多糖,2 mg·kg-1)、灰树花提取物低剂量组(5 mg·kg-1),灰树花提取物中剂量组(10 mg·kg-1)、灰树花提取物高剂量组(20 mg·kg-1),每组5只,滤菌后ip 10 d,第11天处死动物。检测小鼠腹腔巨噬细胞吞噬中性红、产生一氧化氮(NO)能力(Griess法),以及MTT法检测淋巴细胞转化率、自然杀伤细胞(NK细胞)杀伤活性。结果:高剂量灰树花提取物可明显提高小鼠腹腔巨噬细胞吞噬中性红能力(P<0.05)和产生NO能力(P<0.01);高剂量灰树花提取物能明显提高小鼠脾脏B细胞增殖能力(P<0.01);中、高剂量灰树花提取物能明显提高小鼠脾脏T细胞增殖能力(P<0.05和P<0.01)灰树花提取物中、高剂量组小鼠脾脏NK细胞杀伤能力均明显高于脾虚组(P<0.01)。结论:灰树花提取物可以通过提高巨噬细胞吞噬能力和产生NO能力、以及淋巴细胞转化率、NK细胞杀伤活性的途径提高脾虚小鼠的免疫功能。

Effects of Extract of Grifola Frondosa on Peritoneal Macrophages and Spleen Cells in Mouse with Spleen Deficiency

Objective: To investigate the immunological mechanism of extract of grifola frondosa on spleen deficiency mice. Method :Thirty Kunming mice were randomly divided into 6 groups:control group,spleen deficiency group,positive control group and three groups with dosage difference(5,10 and 20 mg ·kg^-1 ).After the establishment of spleen deficiency,the treatment of the extract and the positive drug was lasted for 10 days(ip).Then phagocytosis of peritoneal macrophages was detected by neutral red;NO-producing ability was determined by Griess method;and lymphocyte transformation rate,NK cell activity were tested by MTT method. Result: Extract of grifola frondosa could increase the above mentioned ability. Conclusion: Extract of grifola frondosa can enhance the ability of macrophages and the production of NO,and the lymphocyte transformation rate,NK cell killing activity to improve immunological ability of spleen deficiency in mice.