|
|||||
|
|
| HPLC测定沙苑子中3个黄酮成分 | |
| 引用本文： | 濮延男,唐力英,王祝举,朱洁,崔援军,陈随清.HPLC测定沙苑子中3个黄酮成分[J].中国实验方剂学杂志,2011,17(10):89-91. |
| 作者姓名： | 濮延男 唐力英 王祝举 朱洁 崔援军 陈随清 |
| 作者单位： | 1. 中国中医科学院中药研究所,北京,100700;河南中医学院药学院,郑州450003 2. 中国中医科学院中药研究所,北京,100700 3. 开滦(集团)有限责任公司医疗集团康复医院,河北唐山,063000 4. 河南中医学院药学院,郑州,450003 |
| 摘 要： | 目的:建立沙苑子中3个黄酮的HPLC的含量测定方法。方法:采用高效液相色谱法,Kromasil C18色谱柱(4.6mm×250 mm,5μm),流动相为甲醇-1%冰醋酸(56∶44),流速为1 mL.min-1,柱温30℃,检测波长为266 nm。结果:沙苑子中毛蕊异黄酮、芒柄花素、鼠李柠檬素的线性范围依次为0.348～1.74(r=0.999 7),0.288～1.44(r=0.999 8),0.120～0.60(r=0.999 7),平均回收率(n=5)分别为100.3%(RSD 0.33%),99.8%(RSD 2.78%),98.6%(RSD 2.91%)。结论:该方法操作简便,结果准确,能够同时测定沙苑子药材中3个黄酮的含量,为沙苑子的质量控制和质量标准制定提供了参考依据。 |
| 关 键 词： | 沙苑子 毛蕊异黄酮 芒柄花素 鼠李柠檬素 高效液相色谱法 |
| 收稿时间： | 2/9/2010 12:00:00 AM |
Determination of Three Flavonoids In Seeds of Astragali Complanati by HPLC |
|
| PU Yan-nan,TANG Li-ying,WANG Zhu-ju,ZHU Jie,CUI Yuan-jun and CHEN Sui-qing.Determination of Three Flavonoids In Seeds of Astragali Complanati by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2011,17(10):89-91. | |
| Authors: | PU Yan-nan TANG Li-ying WANG Zhu-ju ZHU Jie CUI Yuan-jun and CHEN Sui-qing |
| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Rehabilitation Hospital of Kailuan Limited Liability Corporation,Tangshan 063000,China;Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China |
| Abstract: | |
| Keywords: | Astragali complanati semen calycosin formononetin rhamnocitrin HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 | |
| 点击此处可从《中国实验方剂学杂志》下载免费的PDF全文 | |