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地耳草HPCE指纹图谱的研究
引用本文:韩乐,宋建平,刘训红,李俊松,蔡宝昌,傅兴圣,陆爱萍.地耳草HPCE指纹图谱的研究[J].中国实验方剂学杂志,2011,17(10):104-107.
作者姓名:韩乐  宋建平  刘训红  李俊松  蔡宝昌  傅兴圣  陆爱萍
作者单位:1. 南京中医药大学,南京,210046
2. 盐城卫生职业技术学院,江苏盐城,224006
基金项目:江苏省中药炮制重点实验室开放式课题
摘    要:目的:建立地耳草毛细管电脉(HPCE)指纹图谱分析方法,评价地耳草药材的质量。方法:用HPCE技术对地耳草进行分析,测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果:初步建立了以6个共有峰为特征指纹信息的HPCE指纹图谱;发现少数样品地耳草HPCE指纹图谱有一定差异。结论:方法准确可靠,重现性好,可作为地耳草内在质量评价的依据。

关 键 词:地耳草  指纹图谱  毛细管电泳
收稿时间:2010/8/21 0:00:00

Studies on HPCE Fingerprint of Herba Hyperici Japonici
HAN Le,SONG Jian-ping,LIU Xun-hong,LI Jun-song,CAI Bao-chang,FU Xing-sheng and LU Ai-ping.Studies on HPCE Fingerprint of Herba Hyperici Japonici[J].China Journal of Experimental Traditional Medical Formulae,2011,17(10):104-107.
Authors:HAN Le  SONG Jian-ping  LIU Xun-hong  LI Jun-song  CAI Bao-chang  FU Xing-sheng and LU Ai-ping
Institution:Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Yancheng Health Vocational & Technical College, Yancheng 224006, China;Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China
Abstract:Objective: To establish the analytical method for the fingerprint of Herba Hyperici Japonici by HPCE and estimate the quality of Herba Hyperici Japonici. Method: Herba Hyperici Japonici were analyzed and the chromatographic fingerprints were determined by HPCE. The data were analyzed by Fuzzy Cluster and Fingerprint Similarity Evaluation Software to compare the similarity of samples. Result: HPCE fingerprint of 6 main peaks was established preliminarily. It was discovered that a small number of samples differed from others. Conclusion: The method is reliable, accurate and can be used for quality control of Herba Hyperici Japonici.
Keywords:Herba Hyperici Japonici  fingerprint  HPCE
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