解酒口服液对乙醇致急性肝损伤的保护作用

目的:研究解酒口服液对乙醇所致急性肝损伤的保护作用。方法:取雄性昆明种小鼠60只,随机分为5组:空白对照组、肝损伤模型对照组、解酒口服液1.67,3.33,10.0 g·kg-1剂量组,ig给药,每日1次,连续30 d。实验末用乙醇给小鼠经口1次ig,造成急性肝损伤模型。用试剂盒检测肝组织中丙二醛(MDA)、还原型谷胱甘肽(GSH)含量,全自动生化分析仪测定肝组织中甘油三酯(TG)的含量,病理检测小鼠肝脏组织脂肪改变的程度。结果:解酒口服液3.33,10.0 g·kg-1剂量组动物的肝脏GSH含量分别为(2.84±0.79),(3.28±0.55)mg.g-1,明显高于模型对照组;解酒口服液10.0 g·kg-1剂量组动物肝脏MDA,TG含量分别为(1.12±0.45)μmol.g-1,(62.4±13.1)μmol.g-1,明显低于模型对照组,差异有统计学意义(P<0.05)。解酒口服液3.33,10.0 g·kg-1剂量组动物的肝脏脂肪改变程度明显低于模型对照组,差异有统计学意义(P<0.05)。结论:解酒口服液对乙醇所致肝损伤具有保护功能。

Protective Action of Jiejiu Oral Liquidon Ethanol-induced Liver Injury in Mice

Objective: To study the protective effect of Jiejiu Oral Liquid on ethanol-induced liver injure. Method: Sixty male KM mice were divided into 5 groups randomly:blank control group, model control group with hepatic injury group and three experimental groups (Jiejiu Oral Liquid 1.67, 3.33,10.0 g ·kg^-1 respectively). Ethanol was given to mice by oral gavage once in order to make the acute liver injury model at the end of experiment. The content of MDA and GSH in hepatic tissue was detected by kits accordingly. The content of TG in hepatic tissue was detected by automatic biochemistry analyzer and the pathological change of fat in hepatic tissue was determined. Result: The content of GSH in 3.33,10.0 g ·kg^-1 groups was significantly higher than that of model control group. The content of MDA and TG was obviously decreased in 10.0 g ·kg^-1 group, compared to that in the hepatic injury model control group ( P <0.05). The pathological change of fat in hepatic tissue in 3.33 and 10.0 g ·kg^-1 groups was significantly less than that of model control group ( P <0.05). Conclusion: Jiejiu Oral Liquid has the function of protecting the liver from ethanol-induced injury.