补肾活血汤对制动应激雌性大鼠生殖内分泌功能紊乱的影响

目的:探讨补肾活血汤对制动应激所致大鼠生殖内分泌功能紊乱的影响。方法:采用重复制动应激法制造大鼠生殖内分泌功能紊乱模型,按8.0,4.0,2.0 g·kg-1分别ig给予补肾活血汤,以放免法检测大鼠血清中性激素:雌二醇(E2)、孕酮(P)、黄体生成素(LH)、卵泡刺激素(FSH)水平;酶免法检测血清中激活素(ACTA)、抑制素(INHB)、卵泡抑素(FS)水平。结果:补肾活血汤8.0,4.0 g·kg-1剂量组可明显升高模型大鼠血清中E2水平,降低异常升高的ACTA水平,升高INHB和FS水平(P<0.05或P<0.01),对生殖内分泌功能紊乱大鼠的卵巢功能有明显的保护作用。结论:补肾活血汤对制动应激所致大鼠生殖内分泌功能紊乱的卵巢功能有明显的保护作用。

Effect of Bushen Huoxuetang on Reproductive-endocrine Disorder Induced by Immobilization Stress in Femail Rats

Objective: To study the effects of Bushen Huoxuetang(BSHXT)in femail rats with Reproductive-endocrine Disorder(RED)induced by immobilization stress. Method :The female rat model of RED was established by repeated immobilization stress. The serum levels of estradiol(E₂), progesterone(P), follicle stimulating hormone(FSH)and luteiuizing hormone(LH)were measured by radioimmunoassay(RIA)and,inhibin-B(INHB), follistatin(FS)and activin-a(ACTA)were measured by enzyme-linked immunosorbent assay(ELISA). Result: The serum level of E₂ was significantly increased and the serum levels of INHB,FS and ACTA were obviously reduced in femail rat model treated by larger and middle doses of BSHXT(8.0,4.0 g ·kg^-1). Conclusion: BSHXT could obviously protect ovarian function of RED induced by immobilization stress in femail rats.