rTMS治疗对实验性家兔脑出血灶周组织单胺类和氨基酸类神经递质变化的影响

目的　探讨重复经颅磁刺激 (rTMS)治疗对脑出血灶周组织单胺类和氨基酸类神经递质变化的影响 ,阐明其作用机制。方法　家兔 5 4只 ,随机分为磁刺激治疗组 (A) 2 4只 ,脑出血模型组 (B) 2 4只 ,对照组 (C) 6只。A、B组采用兔自体血注射 (0 .5ml/kg体重 )制作脑出血模型 ,C组以相同的操作方法注射生理盐水 2ml。A组家兔于造模后 12h实施rTMS ,每日 1次 ,每次 2min ;B组和C组不行rTMS。分别于造模后 12h ,2 4h ,72h和 1周时处死各组动物 ,采用高效液相色谱法检测出血灶周围脑组织中单胺类和氨基酸类神经递质的含量。结果　与C组比较 ,B组谷氨酸 (Glu)和天冬氨酸 (Asp)含量显著增高 ,甘氨酸 (Gly)和γ 氨基丁酸(GABA)含量显著降低 (均P <0 .0 1)。与B组比较 ,A组Glu和Asp含量显著降低 ,Gly和GABA含量显著增高(均P <0 .0 1) ;12h多巴胺 (DA)和 2 4～ 72h 5 羟色胺 (5 HT)含量显著增高 (P <0 .0 1) ,各时间点的去甲肾上腺素 (NA)和肾上腺素 (A)含量变化差异无显著性意义。结论　rTMS刺激可能通过调控兴奋 /抑制性氨基酸递质的平衡、促进单胺类递质的释放 ,在脑出血中发挥治疗作用

Effect of repetitive transcranial magnetic stimulation on contents of monoamine and amino acid transmitters in rabbits with cerebral hemorrhage

Objective To explore the effect of repetitive transcranial magnetic stimulation (rTMS) on contents of monoamine and amino acid transmitters in rabbits with cerebral hemorrhage and the underlying mechanism. MethodsFifty-four rabbits were divided randomly into 3 groups, group A (receiving rTMS) and group B (no rTMS applied) were the experimental groups and group C was the control group. The cerebral hemorrhage model was made by injection of self-body blood, and the control group was made by injection of saline. The rabbits in group A were treated with rTMS once daily and those in groups B and C without rTMS. Rabbits in all 3 groups were executed separately after 12h, 24h, 72h and 1w since the model were established. High performance liquid chromatography (HPLC) method was used to detect the contents of Glu, Asp, Gly, GABA, DA, NA,A and 5-HT in the perihematomal brain tissues. ResultsCompared with group C, the contents of Glu and Asp significantly increased and Gly and GABA significantly decreased, respectively, in group B (P<0.01).Compared with group B, the contents of Glu and Asp significantly decreased and Gly and GABA significantly increased, respectively, (P<0.01),the contents of DA and 5-HT significantly increased in 12h and 24~72h, respectively (P<0.01), while the changes of NA and A were no significant at different time points in group A. ConclusionrTMS could be beneficial for the treatment of cerebral hemorrhage via regulating the balance of the excitatory/inhibitory amino acids to diminishing the excitatory toxic effect, and also promoting the release of monoamine transmitters.