横纹肌肉瘤中MDM2及p53基因表达的原位杂交检测

目的观察ＭＤＭ２、ｐ５３癌基因在横纹肌肉瘤（ＲＭＳ）发病中的作用及其与临床病理、预后间的关系。方法对确诊并有随访的３１例ＲＭＳ标本,用原位杂交技术进行ＭＤＭ２、ｐ５３定位观察。结果发现ＭＤＭ２及ｐ５３癌基因表达阳性率分别为７７４％（２４／３１例）和６６７％（２１／３１例）,其阳性率与年龄、性别和ＲＭＳ组织类型无明显关联（Ｐ＞０．０５）,但阳性率及其强度与ＲＭＳ分化程度（Ⅰ级与Ⅲ级）,转移与否及存活率差异有显著性（Ｐ＜０．０５）。结论ＭＤＭ２、ｐ５３阳性检出率有助于判断ＲＭＳ恶性程度及预测肿瘤的转移和预后。

Detection of MDM 2 and p 53 genes in rhabdomyosarcoma by in situ hybridization

Objective To observe the correlation between MDM2, p53 genes and the pathogenesis, clinical pathology and prognosis in rhabdomyosarcoma (RMS).Methods Thirty one cases of MRS with follow up history were covered for this study. MDM2 and p53 genes were detected by digoxigenin labeling in situ hybridizationtechnique.Results The positive rates for MDM2 and p53 genes were 77.4% (24/31) and 66.7%(21/31) respectively, regardless of age, sex and the histologioal typing of RMS, but there was remarkalbe difference in the positive rates and intensity between the well and poorly differentiated cases, metastastic and non metastic cases as well as one year and three years survival period ( P <0.05).Conclusions The detection of MDM2 and p53 gene is considered valuable in evaluating the maligant degree, predictable metastasis and prognosis of RMS.