| T载体克隆乙肝病毒preS2+S基因的实验研究 |
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| 引用本文： | 张彤,秦东春,张光磊,张绍祖. T载体克隆乙肝病毒preS2+S基因的实验研究[J]. 郑州大学学报(医学版), 1998, 0(1) |
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| 作者姓名： | 张彤 秦东春 张光磊 张绍祖 |
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| 作者单位： | 河南医科大学微生物及免疫学教研室!郑州，450052，河南医科大学第一附属医院检验科!郑州，450052，河南医科大学微生物及免疫学教研室!郑州，450052，河南医科大学微生物及免疫学教研室!郑州，450052 |
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| 摘 要： | 将商品化真核表达载体pcDNA3改造成T载体pcDNA3－T，并运用pcDNA3－T直接克隆了由PCR扩增的adw2亚型乙肝病毒preS2与S基因。结果：pcDNA3－T与PCR产物的重组率高达70％。提示：该方法具有简便，省时等特点。
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| 关 键 词： | T载体 乙肝病毒 preS2＋S基因 真核表达质粒 |
Study on construction of eukaryotic expression plasmid encoding hepatitis B virus preS2+ S gene with T vector |
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| Zhang Tong, Qin Dongchun, Zhang Guanglei, Zhang Shaozu. Study on construction of eukaryotic expression plasmid encoding hepatitis B virus preS2+ S gene with T vector[J]. Journal of Zhengzhou University: Med Sci, 1998, 0(1) |
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| Authors: | Zhang Tong Qin Dongchun Zhang Guanglei Zhang Shaozu |
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| Abstract: | In this study, a commerciallzed eukaryotic expression vector pcDNA3 was modified to produce T vecor pcDNA3 - T,which was directly applied to cloning of preS2 + S gene of adw2 subtype hepatitis B virus.The results showed that pcDNA3-T was efficiently recombined with PCR products, and the novel method was time saving and convenient. |
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| Keywords: | T vector hepatitis Bvirus pres2 + S gene eukaryotic expression plasmid |
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