荧光定量PCR检测HBV-DNA与乙肝两对半检测结果的相关性探讨

目的:探讨HBV-DNA的检出情况与乙肝两对半结果之间的关系。方法:运用荧光定量PCR(FQ-PCR)检测307例疑似乙肝患者血清中的HBV-DNA,同时运用ELISA方法进行两对半指标的检测,并对结果进行相关性探讨。结果:HBsAg( )HBeAg( )HBcAb( )组(大三阳组)患者血清HBV-DNA检出率为98.15%(53/54),平均拷贝数为7.56E 06/m l;HBsAg( )HBeAb( )HBcAb( )组(小三阳组)患者血清HBV-DNA检出率为27.78%(15/54),平均拷贝数为3.79E 05/m l;HBsAg( )HBcAb( )组血清HBV-DNA检出率为91.18%(31/34),平均拷贝数为2.85E 05/m l,小三阳组HBV-DNA阳性检出率及拷贝数均低于大三阳组,且与大三阳组比较均具有显著性差异(P<0.01,P<0.05);HBsAb( )组血清HBV-DNA检出率为1.6%(1/63),平均拷贝数为1.13E 08/m l;HBcAb( )组血清HBV-DNA检出率为16.67%(1/6),平均拷贝数为3.00E 04/m l;HBsAb( )HBeAb( )HBcAb( )组血清HBV-DNA检出率为12.50%(4/32),平均拷贝数为3.00E 05/m l;HBsAg( )组血清HBV-DNA检出率为0(0/2);HBV-M全阴性组HBV-DNA检出率为1.61%(1/62),平均拷贝数为2.75E 05/m l。结论:HBV-M阴性患者仍有HBV-DNA阳性者,因此在检测中应联合应用两种方法进行检测,提高检出率,避免漏诊,为临床HBV感染、复制及传染性的判断以及指导治疗提供更为可靠的判定依据。

Correlation of Detection of HBV-DNA by Fluorescent Quantitative PCR with Routine Detection of HBV

Objective:To explore the relationship between detection of HBV-DNA and routine detection of HBV.Method:HBV-DNA was detected with fluorescent quantitative PCR(FQ-PCR) and HBV was routinely determined with ELISA in serum samples from 307 suspected cases of hepatitis B.Meanwhile,the correlation of results between the two methods was analyzed.Result:The detecting rate of HBV-DNA was 98.15%(53/54) and the mean copy number was 7.56E 06/ml in patients of HBsAg( ),HBeAg( ) and HBcAb( )(group A),27.78%(15/54)and 3.79E 05/ml in patients of HBsAg( ),HBeAb( ) and HBcAb( )(group B),91.18%(31/34)and 2.85E 05/ml in patients of HBsAg( ) and HBcAb( )(group C).The 2 parameters were significantly lower in group B than in group A(P<0.01 and 0.05).Furthermore,they were respectively 1.6%(1/63) and 1.13E 08/ml in patients of HBsAb( ),16.67%(1/6) and 3.00E 04/ml in patients of HBcAb( ),12.50%(4/32) and 3.00E 05/ml in patients of HBsAb( ),HBeAb( ) and HBcAb( ),0(0/2) in patients of HBsAg( ) and 1.61%(1/62)and 2.75E 05/ml in HBV-M-negative patients.Conclusion:HBV-DNA positivity may appear in HBV-M-negative patients.Therefore,the 2 methods should be simultaneously used in the detection of HBV infection to promote the detecting rate to provide more reliable evidence for judgment of HBV infection,duplication and infectivity and its treatment in clinical practice.