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| 反义寡核苷酸抑制血管平滑肌细胞增殖 | |
| 引用本文： | 边杰芳,张柏根,钱虎声,陈诗书. 反义寡核苷酸抑制血管平滑肌细胞增殖[J]. 医学争鸣, 2000, 21(12): 1492-1494 |
| 作者姓名： | 边杰芳 张柏根 钱虎声 陈诗书 |
| 作者单位： | 1. 第四军医大学西京医院血管外科,陕西,西安,710033 2. 上海第二医科大学仁济医院心血管外科 3. 上海第二医科大学分子生物学实验室和人类基因治疗研究中心 |
| 基金项目： | 国家自然科学基金资助项目! (3 9470 681) |
| 摘 要： | 目的 证实反义ｃ－ｍｙｃ，ＰＣＮＡ寡核苷酸阻遏血管平滑肌细胞（ＶＳＭＣ）增殖的效应。方法 人工合成正义、反义及错配反义ｃ－ｍｙｃ，ＰＣＮＡ寡核苷酸，转入体外培养的ＶＳＭＣ中，分别于作用后１～５ｄ进行细胞计数和＾３Ｈ－ＴｄＲ掺入法测定。结果加入反义ｃ－ｍｙｃ，ＰＣＮＡ寡核苷酸的ＶＳＭＣ增殖受到抑制，崦正义和错配反义寡核苷酸对ＶＳＭＣ增殖没有明显影响。结论 反义ｃ－ｍｙｃ，ＰＣＮＡ寡核苷酸可有效阻遏 |
| 关 键 词： | 反义寡核苷酸 血管平滑肌 细胞计理技术 |
| 修稿时间： | : |
Antisense oligonucleotide inhibits proliferation of vascular smooth muscle cells |
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| BIAN Jie-Fang,ZHANG Bai-Gen,QIAN Hu-Sheng,CHEN Shi-Shu. Antisense oligonucleotide inhibits proliferation of vascular smooth muscle cells[J]. Negative, 2000, 21(12): 1492-1494 | |
| Authors: | BIAN Jie-Fang ZHANG Bai-Gen QIAN Hu-Sheng CHEN Shi-Shu |
| Abstract: | AIM To study the effect of antisense c-myc and proliferating cell nuclear antigen(PCNA) oligonucleotides on proliferation of vascular smooth muscle cell(VSMC) in vitro. METHODS Antisense, sense, and mismatched oligonucleotides targeting c-myc or PCNA were synthesized, and individually delivered to cultural VSMC. After 1 to 5 days, the cell number was counted and the [3H] thymidine incorporation measured. RESULTS Antisense oligonucleotides could inhibit the VSMC proliferation, while the sense and the mismatched oligonucleotides did not have this effect. CONCLUSION Antisense c-myc and PCNA are inhibitors of VSMC proliferation. |
| Keywords: | antisense oligonucleotide smooth muscle vascular cytometry protooncogene |
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